Document Detail

Effect of hydrogen peroxide on amino acid concentrations in bovine retina and vitreous humor, ex vivo.
MedLine Citation:
PMID:  14685296     Owner:  NLM     Status:  MEDLINE    
In a previous study, we showed evidence that oxidative stress induced by hydrogen peroxide (H2O2) can inhibit the release of [3H]-D-aspartate from the bovine isolated retina, in vitro. The aim of the present study was to investigate the effect of H2O2 on glutamate and glycine levels in the bovine retina and vitreous humor, ex vivo. Furthermore, we examined whether inhibition of catalase activity with 3-amino-triazole had any effect on the concentrations of these amino acids in the posterior segment of the bovine eye. Whole eye organ cultures were prepared by incubating tissues in oxygenated Krebs solution at 37 masculine C for 30 min. After incubation, H2O2 (1-100 microM) or sterile distilled water was injected intravitreally into each eye. Thirty minutes after injection, the retina and vitreous humor were removed for analysis of glutamate and glycine by high performance liquid chromatography (HPLC) with fluorescence detection. Exogenously applied H2O2 (1-100 microM) caused a concentration-related decrease in both glutamate and glycine levels in the bovine retina. Furthermore, while H2O2 (1-10 microM) caused a concentration-dependent decrease in glycine levels in the vitreous humor, it had no significant effect on glutamate levels. The catalase inhibitor, 3-amino-triazole (10 mM), caused a significant reduction in both glutamate and glycine levels in the bovine retina, ex vivo. Likewise, 3-AT caused an attenuation in both glutamate and glycine concentration in the vitreous humor. We conclude that oxidative stress induced by H2O2 can alter the release and/or availability of amino acids in the posterior segment of bovine eyes.
A M LeDay; S Ganguly; K H Kulkarni; A Dash; C A Opere; S E Ohia
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Methods and findings in experimental and clinical pharmacology     Volume:  25     ISSN:  0379-0355     ISO Abbreviation:  Methods Find Exp Clin Pharmacol     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-12-19     Completed Date:  2004-08-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7909595     Medline TA:  Methods Find Exp Clin Pharmacol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  695-701     Citation Subset:  IM    
Department of Pharmacy Sciences, School of Pharmacy and Allied Health Professions, Creighton University, Omaha, Nebraska, USA.
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MeSH Terms
Amino Acids / analysis,  metabolism*
Dose-Response Relationship, Drug
Hydrogen Peroxide / pharmacology*
Organ Culture Techniques
Retina / chemistry,  drug effects*,  metabolism
Vitreous Body / chemistry,  drug effects*,  metabolism
Grant Support
Reg. No./Substance:
0/Amino Acids; 7722-84-1/Hydrogen Peroxide

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