| Effect of hydrazine upon vitamin B12-dependent methionine synthase activity and the sulphur amino acid pathway in isolated rat hepatocytes. | |
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MedLine Citation:
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PMID: 10230775 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of the industrial chemical, hydrazine (4-12 mM), on methionine synthase (EC 2.1.1.13) activity and levels of the sulphur amino acids homocysteine, cysteine, and taurine as well as GSH were investigated in vitro in isolated rat hepatocyte suspensions and monolayers in order to explain some of the adverse in vivo effects of hydrazine. None of the concentrations of hydrazine were overtly cytotoxic in hepatocyte suspensions (measured as lactate dehydrogenase [LDH] leakage) after 3 hr. However, after 24 hr in culture cells treated with 12 mM, hydrazine showed a significant increase in LDH leakage. Methionine synthase activity was reduced by hydrazine (8 and 12 mM) in suspensions (by 45 and 55%, after 3 hr) and monolayers (12 mM; 65-80% after 24 hr). This was not due to nitric oxide production and the inhibitor of nitric oxide synthase, Nomega-nitro-L-arginine, failed to protect against the hydrazine-induced loss of ATP and GSH and the reduction in urea synthesis at 24 hr. Homocysteine export was increased by 6 mM hydrazine, and total taurine content of treated cells was increased by 12 mM hydrazine. Thus, hydrazine was found to have several important and possibly deleterious effects on some parts of the sulphur amino acid pathway. |
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Authors:
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S H Kenyon; C J Waterfield; D S Asker; M Kudo; D W Moss; T E Bates; A Nicolaou; W A Gibbons; J A Timbrell |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical pharmacology Volume: 57 ISSN: 0006-2952 ISO Abbreviation: Biochem. Pharmacol. Publication Date: 1999 Jun |
Date Detail:
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Created Date: 1999-06-01 Completed Date: 1999-06-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0101032 Medline TA: Biochem Pharmacol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1311-9 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutical and Biological Chemistry, School of Pharmacy, University of London, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
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metabolism* Amino Acids, Sulfur / metabolism* Animals Carcinogens / pharmacology Cell Survival / drug effects Culture Media / metabolism Cysteine / metabolism Cytosol / metabolism Enzyme Inhibitors / pharmacology Homocysteine / metabolism Hydrazines / pharmacology* Liver Male Nitroarginine / pharmacology Rats Rats, Wistar Taurine / metabolism Vitamin B 12 / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids, Sulfur; 0/Carcinogens; 0/Culture Media; 0/Enzyme Inhibitors; 0/Hydrazines; 107-35-7/Taurine; 2149-70-4/Nitroarginine; 302-01-2/hydrazine; 454-28-4/Homocysteine; 52-90-4/Cysteine; 68-19-9/Vitamin B 12; EC 2.1.1.13/5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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