Document Detail


Effect of human umbilical cord blood-derived mesenchymal stem cells in a cirrhotic rat model.
MedLine Citation:
PMID:  19422480     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIM: Cirrhosis is a long-term consequence of chronic hepatic injury and no effective therapy is currently available for this disease. Recent reports have shown that the mesenchymal stem cells (MSCs) have the capacity to differentiate into hepatocytes, and umbilical cord blood is a rich source of MSCs. Hence, we investigated the effect of infusing of human umbilical cord blood-derived MSCs (HMSCs) in carbon tetrachloride (CCl4)-induced cirrhosis in a rat model. METHODS: The effect of HMSCs on cirrhosis was evaluated using haematoxylin and eosin and Masson's trichrome staining. To evaluate cirrhosis-related factors, we measured protein and mRNA expression of transforming growth factor beta1 (TGF-beta1), collagen type I and alpha-smooth muscle actin (alpha-SMA). RESULTS: Histological findings showed that liver fibrosis in rats was alleviated by HMSCs infusion. Interestingly, CM-DiI-labelled HMSCs expressed the hepatocyte-specific markers, human albumin and alpha-fetoprotein. Infusion of HMSCs significantly inhibited TGF-beta1, collagen type I and alpha-SMA expressions in CCl4-induced cirrhotic rats. CONCLUSION: Our results showed that HMSCs infusion could improve liver fibrosis in rats with CCl4-induced cirrhosis, raising the possibility for clinical use of HMSCs in the treatment of cirrhosis.
Authors:
Kyung Hee Jung; Hyun Phil Shin; Sun Lee; Yun Jeong Lim; Soo Han Hwang; Hoon Han; Hwon Kyum Park; Joo-Ho Chung; Sung-Vin Yim
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-17
Journal Detail:
Title:  Liver international : official journal of the International Association for the Study of the Liver     Volume:  29     ISSN:  1478-3231     ISO Abbreviation:  Liver Int.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-29     Completed Date:  2009-10-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101160857     Medline TA:  Liver Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  898-909     Citation Subset:  IM    
Affiliation:
Kohwang Medical Research Institute, School of Medicine, Kyung Hee University, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
Blotting, Western
Carbon Tetrachloride
Fetal Blood / cytology*
Humans
Immunohistochemistry
In Situ Hybridization, Fluorescence
Liver / pathology
Liver Cirrhosis, Experimental / therapy*
Mesenchymal Stem Cell Transplantation
Mesenchymal Stem Cells / physiology*
Rats
Reverse Transcriptase Polymerase Chain Reaction
Tissue Therapy / methods*
Transforming Growth Factor beta1 / metabolism
Chemical
Reg. No./Substance:
0/Actins; 0/Tgfb1 protein, rat; 0/Transforming Growth Factor beta1; 0/smooth muscle actin, rat; 56-23-5/Carbon Tetrachloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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