Document Detail

Effect of human embryonic stem cell-derived neuronal precursor cell transplantation into the cerebral infarct model of rat with exercise.
MedLine Citation:
PMID:  17408791     Owner:  NLM     Status:  MEDLINE    
We analyzed the therapeutic effect of the transplantation of the human embryonic stem cell (NIH Code: MB01)-derived neuronal precursor (hES-NP) cell and post-ischemic exercise in rats with the middle cerebral artery (MCA) infarct model. A cortical infarct was induced in 20 adult Sprague-Dawley rats by occlusion and reperfusion of the MCA. The rats were divided into four groups: hES-NP cell transplantation and exercise, transplantation only, exercise only, and Sham-operated with no exercise. In the cell-transplanted group, hES-NP cells were transplanted by stereotactic inoculation into the ipsilateral basal ganglia 7 days after infarct. We evaluated the clinical recovery of deficit, the size of infarct and the survival, migration, and differentiation of the transplanted cells. The transplanted hES-NP cells survived robustly in the ischemic brains 3 weeks post transplant. The majority of migrating cells in the ischemic rats had a neuronal phenotype. The clinical scores of all of the experimental groups were better than those of the Sham-operated group. Whereas the exercise-only group showed continuous clinical improvement, the cell-transplanted groups manifested less improvement than the exercise-only group. Moreover, the cell-transplanted groups did not differ in clinical improvement according to postinfarct-exercise or not. The infarct size was significantly reduced in both the cell-transplanted groups and the post-ischemic exercise group, compared with the Sham-operated group; however, the reduction of infarct size was most prominent in the exercise-only group. In our study, the inoculated site of the basal ganglia showed some damage induced by inoculation, such as loss of neuroglial cells, reactive gliosis and microcalcification, which was found in the Sham-operated group as well, and yet no inoculation-site injury has ever been reported. Our study revealed that stem cell transplantation can have a positive effect on behavioral recovery and reduction of infarct size, but the effect shown was no better than the effect of the exercise, which finding reconfirmed the importance of post-infarct rehabilitation. In addition, it was found that cell inoculation should be replaced by a noninvasive procedure.
Dae-Yul Kim; Sung-Hye Park; Si-Uk Lee; Deok-Hyung Choi; Hee-Won Park; Sun Ha Paek; Hye Young Shin; Eun-Young Kim; Se-Pill Park; Jin Ho Lim
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-03-01
Journal Detail:
Title:  Neuroscience research     Volume:  58     ISSN:  0168-0102     ISO Abbreviation:  Neurosci. Res.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-05     Completed Date:  2007-11-01     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8500749     Medline TA:  Neurosci Res     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  164-75     Citation Subset:  IM    
Department of Rehabilitation Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.
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MeSH Terms
Disease Models, Animal
Embryonic Stem Cells / physiology*
Infarction, Middle Cerebral Artery / pathology,  rehabilitation*,  surgery*
Motor Activity / physiology
Nerve Tissue Proteins / metabolism
Physical Conditioning, Animal / methods*
Proto-Oncogene Proteins c-kit / metabolism
RNA, Messenger / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction / methods
Statistics, Nonparametric
Stem Cell Transplantation / methods*
Time Factors
Transplantation, Heterologous / methods
Reg. No./Substance:
0/Nerve Tissue Proteins; 0/RNA, Messenger; EC Proteins c-kit

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