| Effect of high soy diet on the cerebrovasculature and endothelial nitric oxide synthase in the ovariectomized rat. | |
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MedLine Citation:
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PMID: 20197113 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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High soy (HS) diets are neuroprotective and promote vascular dilatation in the periphery. We hypothesized that an HS diet would promote vascular dilatation in the cerebrovasculature by mimicking estradiol's actions on the endothelial nitric oxide synthase (eNOS) system including increasing eNOS expression and decreasing caveolin-1 expression to increase nitric oxide (NO) production. Ovariectomized rats were fed HS or a soy-free diet (SF)+/-low physiological estradiol (E2) for 4weeks. Neither E2 nor HS altered middle cerebral artery (MCA) structure or vascular responses to acetylcholine, serotonin, or phenylephrine. Estradiol enhanced bradykinin-induced relaxation in an eNOS-dependent manner. Although E2 and HS increased eNOS mRNA expression in the brain and cerebrovasculature, they had no effect on eNOS protein expression or phosphorylation in the MCA. However, E2 decreased caveolin-1 protein in the MCA. In MCAs neither E2 nor HS altered estrogen receptor (ER) alpha expression, but E2 did reduce ER beta levels. These data suggest that HS diets have no effect on vascular NO production, and that E2 may modulate basal NO production by reducing the expression of caveolin-1, an allosteric inhibitor of NOS activity. However, the effects of E2 and HS on the cerebrovasculature are small and may not underlie their protective actions in pathological states. |
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Authors:
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Derek A Schreihofer; Christian Deutsch; Tara Lovekamp-Swan; Jennifer C Sullivan; Anne M Dorrance |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-03-01 |
Journal Detail:
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Title: Vascular pharmacology Volume: 52 ISSN: 1879-3649 ISO Abbreviation: Vascul. Pharmacol. Publication Date: 2010 May-Jun |
Date Detail:
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Created Date: 2010-04-23 Completed Date: 2010-08-02 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 101130615 Medline TA: Vascul Pharmacol Country: United States |
Other Details:
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Languages: eng Pagination: 236-42 Citation Subset: IM |
Affiliation:
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Department of Physiology, Medical College of Georgia, Augusta, GA 30912-3000, USA. dschreihofer@mcg.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Caveolin 1 / drug effects, genetics Diet Estradiol / pharmacology Estrogen Receptor beta / drug effects, metabolism Female Gene Expression Regulation, Enzymologic / drug effects Isoflavones / administration & dosage, pharmacology* Middle Cerebral Artery / drug effects, metabolism Nitric Oxide / metabolism Nitric Oxide Synthase Type III / drug effects*, genetics, metabolism Ovariectomy RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Soybeans / chemistry* Vasodilation / drug effects* |
| Grant Support | |
ID/Acronym/Agency:
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NIH RO1 HL0077385/HL/NHLBI NIH HHS; R01 AT001882-04/AT/NCCAM NIH HHS; R01 HL077385-01/HL/NHLBI NIH HHS; R01AT001882/AT/NCCAM NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Caveolin 1; 0/Estrogen Receptor beta; 0/Isoflavones; 0/RNA, Messenger; 10102-43-9/Nitric Oxide; 50-28-2/Estradiol; EC 1.14.13.39/Nitric Oxide Synthase Type III |
| Comments/Corrections | |
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