Document Detail

Effect of hepatocytes from normal or endotoxin treated animals on the production of tumour necrosis factor, interleukin-6, and prostaglandin-E2 by macrophages incubated in vitro with various fatty acids.
MedLine Citation:
PMID:  7772629     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To find out if the in vitro production of tumour necrosis factor (TNF), interleukin 6 (IL-6) and prostaglandin E2 (PGE2) by macrophages (P3888D1) were influenced by the addition to the culture media of hepatocytes from normal guinea pigs and from guinea pigs after infusion with endotoxin with and without fatty acids. DESIGN: Laboratory experiment. SETTING: University hospital, United States MATERIAL: 12 male Hartley guinea pigs. MAIN OUTCOME MEASURES: Concentrations of TNF-alpha, IL-6, and PGE2. RESULTS: Hepatocytes inhibited the production of TNF by macrophages. Those from animals pretreated with endotoxin caused a large increase in the production of PGE2 and affected the production of IL-6 only in the presence of fatty acids. Fatty acids differed from each other in their effects on production of TNF-alpha and PGE2, but had no effect on the production of IL-6. CONCLUSION: These results support the concept that hepatocytes and macrophages may interact in vivo, and this interaction may be influenced both by fatty acids that arise from lipid metabolism and from endotoxin present during sepsis and translocation.
C K Ogle; X Guo; J Z Wu; J D Ogle; J E Fischer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The European journal of surgery = Acta chirurgica     Volume:  161     ISSN:  1102-4151     ISO Abbreviation:  Eur J Surg     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-07-13     Completed Date:  1995-07-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9105264     Medline TA:  Eur J Surg     Country:  NORWAY    
Other Details:
Languages:  eng     Pagination:  123-7     Citation Subset:  IM    
Department of Surgery, University of Cincinnati, College of Medicine, Ohio, USA.
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MeSH Terms
Blood Proteins / pharmacology*
Cell Line
Cells, Cultured
Culture Media
Cytokines / biosynthesis
Dinoprostone / biosynthesis
Fatty Acids, Unsaturated / pharmacology
Guinea Pigs
Interleukin-6 / pharmacology*
Liver / cytology*,  drug effects
Macrophages / drug effects*
Tumor Necrosis Factor-alpha / biosynthesis*,  drug effects
Grant Support
Reg. No./Substance:
0/Blood Proteins; 0/Cardenolides; 0/Culture Media; 0/Cytokines; 0/Fatty Acids, Unsaturated; 0/Interleukin-6; 0/Saponins; 0/Tumor Necrosis Factor-alpha; 0/digoxin-like factors; 20830-75-5/Digoxin; 363-24-6/Dinoprostone

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