| Effect of a hepatocyte growth factor/heparin-immobilized collagen system on albumin synthesis and spheroid formation by hepatocytes. | |
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MedLine Citation:
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PMID: 20547342 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A hepatocyte growth factor (HGF)/heparin-immobilized collagen system was used as a synthetic extracellular matrix for hepatocyte culture. The albumin synthesis, nucleus numbers and morphology of the hepatocytes were determined separately to evaluate the hepatocyte number and hepatocyte-specific function under this system. The benefits of the HGF/heparin-immobilized collagen system for hepatocyte culture were confirmed by three types of culture methods in vitro, namely 2D film cultures, 2D gel cultures and 3D gel cultures. In 2D collagen film cultures, hepatocytes exhibited the highest albumin synthesis (1.42 microg/well/day) in HGF/heparin-immobilized collagen films at 7 days of culture. Heparin inhibited hepatocyte adhesion while HGF promoted hepatocyte migration, and spheroid formation was easily detected in HGF/heparin-immobilized collagen films. In 2D collagen gel cultures, albumin synthesis of around 15 microg/well/day was detected and maintained for more than 18 days on HGF/heparin-immobilized collagen gels. Similar findings were obtained in 3D HGF/heparin-immobilized collagen gel cultures, which exhibited albumin synthesis of up to 30 microg/well/day. The albumin synthesis by hepatocytes was two-fold higher in 3D gel cultures compared with 2D gel cultures, and was maintained for over 2 weeks compared with 2D film cultures using the HGF/heparin-immobilized collagen system. Taken together, the HGF/heparin-immobilized collagen system was effective for albumin synthesis by hepatocytes in both 2D film cultures and 3D gel cultures, and therefore shows good potential for tissue engineering use. |
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Authors:
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Yung-Te Hou; Hiroyuki Ijima; Shunichi Matsumoto; Takafumi Kubo; Takayuki Takei; Shinji Sakai; Koei Kawakami |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-13 |
Journal Detail:
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Title: Journal of bioscience and bioengineering Volume: 110 ISSN: 1347-4421 ISO Abbreviation: J. Biosci. Bioeng. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-06 Completed Date: 2010-12-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888800 Medline TA: J Biosci Bioeng Country: Japan |
Other Details:
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Languages: eng Pagination: 208-16 Citation Subset: IM |
Copyright Information:
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Crown Copyright 2010. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, 744 Motooka, Nishi-Ku, Fukuoka, 819-0395, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anticoagulants / administration & dosage, chemistry Cell Enlargement / drug effects Cell Proliferation / drug effects Cells, Cultured Collagen / administration & dosage*, chemistry Heparin / administration & dosage*, chemistry Hepatocyte Growth Factor / administration & dosage*, chemistry Hepatocytes / drug effects, physiology* Liver, Artificial* Male Protein Binding Rats Rats, Wistar Serum Albumin / biosynthesis* Spheroids, Cellular / drug effects, physiology* Tissue Engineering / methods |
| Chemical | |
Reg. No./Substance:
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0/Anticoagulants; 0/Serum Albumin; 67256-21-7/Hepatocyte Growth Factor; 9005-49-6/Heparin; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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