Document Detail

Effect of heparin on the utilization in vitro of labelled glycerides from triglyceride-rich lipoproteins in rat adipose tissue.
MedLine Citation:
PMID:  6163408     Owner:  NLM     Status:  MEDLINE    
Triglyceride-rich lipoproteins from adult rat plasma were labelled in vivo with 3H in the esterified fatty acids and 14C in the labelled glyceride glycerol of neutral lipids by injecting i.v. sodium 9-10 (n)-[3H] palmitate and [U-14C] glycerol, after which the prelabelled lipoproteins were purified by ultracentrifugation and dialysis. The lipoproteins were incubated in vitro, in the presence or not of heparin, with pieces of epididymal fat pads or isolated adipocytes from fed rats. The disappearance of both [3H]- and [14C] lipids from the media was greater when incubations were performed with adipocytes than with fat-pad pieces and it increased with heparin in both preparations. More 3H-label than 14C was found in the tissue lipids, a higher percentage being present in adipocytes than in fat-pad pieces, and the amount of label in tissue lipids was always enhanced by heparin. Some 14C-label appeared as esterified fatty acids in both tissue preparations and it also was enhanced by the presence of heparin. These findings are in agreement with the recognized influence of heparin on the release of lipoprotein lipase and show the direct relationship between heparin action and tissue ability to take up products of lipoprotein triglyceride breakdown. They also demonstrate the ability of adipose tissue to metabolize glycerol coming from the hydrolysis of lipoprotein glycerides.
M A Lasunción; E Herrera
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives internationales de physiologie et de biochimie     Volume:  88     ISSN:  0003-9799     ISO Abbreviation:  Arch. Int. Physiol. Biochim.     Publication Date:  1980 Oct 
Date Detail:
Created Date:  1981-05-21     Completed Date:  1981-05-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0405355     Medline TA:  Arch Int Physiol Biochim     Country:  BELGIUM    
Other Details:
Languages:  eng     Pagination:  385-91     Citation Subset:  IM    
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MeSH Terms
Adipose Tissue / drug effects,  metabolism*
Carbon Radioisotopes
Fatty Acids / biosynthesis
Fatty Acids, Nonesterified / biosynthesis
Glycerol / metabolism
Heparin / pharmacology*
Lipids / biosynthesis
Lipoproteins / metabolism*
Palmitates / metabolism
Triglycerides / metabolism*
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Fatty Acids; 0/Fatty Acids, Nonesterified; 0/Lipids; 0/Lipoproteins; 0/Palmitates; 0/Triglycerides; 10028-17-8/Tritium; 56-81-5/Glycerol; 9005-49-6/Heparin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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