Document Detail

Effect of heparin on apoptosis in human nasopharyngeal carcinoma CNE2 cells.
MedLine Citation:
PMID:  11787776     Owner:  NLM     Status:  MEDLINE    
In order to study the mechanism of the effect of heparin on apoptosis in carcinoma cells, the nasopharyngeal carcinoma cell line CNE2 was used to identify the effect of heparin on apoptosis associated with the expression of c-myc, bax, bcl-2 proteins by use of Hoechst 33258 staining, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL), agarose gel electrophoresis, and flow cytometry, as well as Western blot analysis. The results showed that heparin induced apoptosis of CNE2 cells including the morphologic changes such as reduction in the volume, and the nuclear chromatin condensation, as well as the "ladder pattern" revealed by agarose gel electrophoresis of DNA in a concentration-dependent manner. The number of TUNEL-positive cells was dramatically increased to 33.6+/-1.2% from 2.8+/-0.3% by treatment with heparin in different concentrations (10 to approximately 40 kU/L). The apoptotic index was increased to 32.5% from 3.5% by detecting SubG1 peaks on flow cytometry. Western blot analysis showed that levels of bcl-2, bax and c-myc were significantly overexpressed by treatment with the increase of heparin concentrations. These results suggest that heparin induces apoptosis of CNE2 cells, which may be regulated by differential expression of apoptosis-related genes.
H L Li; K H Ye; H W Zhang; Y R Luo; X D Ren; A H Xiong; R Situ
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell research     Volume:  11     ISSN:  1001-0602     ISO Abbreviation:  Cell Res.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2002-01-11     Completed Date:  2002-08-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9425763     Medline TA:  Cell Res     Country:  China    
Other Details:
Languages:  eng     Pagination:  311-5     Citation Subset:  IM    
Department of Pharmacology, Pharmacy College, Jinan University, Guangzhou, China.
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MeSH Terms
Antineoplastic Agents / pharmacology*
Carcinoma / metabolism,  pathology*,  ultrastructure
Heparin / pharmacology*
Nasopharyngeal Neoplasms / metabolism,  pathology*,  ultrastructure
Proto-Oncogene Proteins / biosynthesis
Proto-Oncogene Proteins c-bcl-2 / biosynthesis
Proto-Oncogene Proteins c-myc / biosynthesis
Tumor Cells, Cultured
bcl-2-Associated X Protein
Reg. No./Substance:
0/Antineoplastic Agents; 0/BAX protein, human; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Proto-Oncogene Proteins c-myc; 0/bcl-2-Associated X Protein; 9005-49-6/Heparin

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