Document Detail


Effect of hemorrhage on medial collateral ligament healing in a mouse model.
MedLine Citation:
PMID:  12975183     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Medial collateral ligament injuries heal by a scar response. HYPOTHESIS: Increased hemorrhage at the site of medial collateral ligament injury improves healing. STUDY DESIGN: Controlled laboratory study. METHODS: Ninety-six mice were divided into two groups. Group 1 mice underwent knee medial collateral ligament transection with the opposite knee as a sham-operated control and group 2 animals additionally had 0.25 ml of tail cut blood pipetted to the medial collateral ligament transection site and sham-operated opposite knee. Ligament specimens were harvested at 3, 7, 21, and 28 days. RESULTS: Immunohistochemical analysis demonstrated peak macrophage counts at day 7 in all transected specimens. Macrophage counts were higher in group 2 than in group 1 at all time points, with a statistically significant increase of macrophages noted at day 7. In situ hybridization demonstrated increased collagen gene expression, with peaks at 7 and 28 days after transection. Group 2 animals showed increased gene expression at all time points as compared with group 1, with a statistically significant increase noted at 7 and 28 days. Biomechanical testing demonstrated progressive healing at each time point. At 28 days, the load to failure was 67% that of the sham-operated knee. CONCLUSIONS: This study suggests there is an increased healing response with bleeding at the ligament injury site. Clinical Relevance: Identification of the factors involved with increased healing may allow manipulation of the healing response in the clinical setting.
Authors:
Rick W Wright; Manish Parikh; Tracy Allen; Michael D Brodt; Matthew J Silva; Mitchell D Botney
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of sports medicine     Volume:  31     ISSN:  0363-5465     ISO Abbreviation:  Am J Sports Med     Publication Date:    2003 Sep-Oct
Date Detail:
Created Date:  2003-09-16     Completed Date:  2004-03-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7609541     Medline TA:  Am J Sports Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  660-6     Citation Subset:  IM    
Affiliation:
Department of Orthopaedic Surgery, Washington University School of Medicine at Barnes-Jewish Hospital, St Louis, Missouri 63110, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biomechanics
Collagen / biosynthesis
Disease Models, Animal
Gene Expression Regulation
Hemorrhage*
Immunohistochemistry
Macrophages
Medial Collateral Ligament, Knee / pathology,  surgery*
Mice
Wound Healing
Chemical
Reg. No./Substance:
9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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