| Effect of hemorrhage on medial collateral ligament healing in a mouse model. | |
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MedLine Citation:
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PMID: 12975183 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Medial collateral ligament injuries heal by a scar response. HYPOTHESIS: Increased hemorrhage at the site of medial collateral ligament injury improves healing. STUDY DESIGN: Controlled laboratory study. METHODS: Ninety-six mice were divided into two groups. Group 1 mice underwent knee medial collateral ligament transection with the opposite knee as a sham-operated control and group 2 animals additionally had 0.25 ml of tail cut blood pipetted to the medial collateral ligament transection site and sham-operated opposite knee. Ligament specimens were harvested at 3, 7, 21, and 28 days. RESULTS: Immunohistochemical analysis demonstrated peak macrophage counts at day 7 in all transected specimens. Macrophage counts were higher in group 2 than in group 1 at all time points, with a statistically significant increase of macrophages noted at day 7. In situ hybridization demonstrated increased collagen gene expression, with peaks at 7 and 28 days after transection. Group 2 animals showed increased gene expression at all time points as compared with group 1, with a statistically significant increase noted at 7 and 28 days. Biomechanical testing demonstrated progressive healing at each time point. At 28 days, the load to failure was 67% that of the sham-operated knee. CONCLUSIONS: This study suggests there is an increased healing response with bleeding at the ligament injury site. Clinical Relevance: Identification of the factors involved with increased healing may allow manipulation of the healing response in the clinical setting. |
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Authors:
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Rick W Wright; Manish Parikh; Tracy Allen; Michael D Brodt; Matthew J Silva; Mitchell D Botney |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of sports medicine Volume: 31 ISSN: 0363-5465 ISO Abbreviation: Am J Sports Med Publication Date: 2003 Sep-Oct |
Date Detail:
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Created Date: 2003-09-16 Completed Date: 2004-03-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7609541 Medline TA: Am J Sports Med Country: United States |
Other Details:
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Languages: eng Pagination: 660-6 Citation Subset: IM |
Affiliation:
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Department of Orthopaedic Surgery, Washington University School of Medicine at Barnes-Jewish Hospital, St Louis, Missouri 63110, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biomechanics Collagen / biosynthesis Disease Models, Animal Gene Expression Regulation Hemorrhage* Immunohistochemistry Macrophages Medial Collateral Ligament, Knee / pathology, surgery* Mice Wound Healing |
| Chemical | |
Reg. No./Substance:
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9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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