| Effect of hemodialysis on the content of fatty acids in monolayers of erythrocyte membranes in patients with chronic renal failure. | |
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MedLine Citation:
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PMID: 17497467 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lipid metabolism disorders are found in patients with chronic renal failure (CRF). Changes in the content of fatty acids of the phospholipid fraction of erythrocyte membranes can lead to changes in the rheological properties. The objective of our study was to assess the effect of hemodialysis on the composition of fatty acids in two fractions of phospholipids: sphingomyelin (SPH, representative of the external monolayer) and phosphatidylethanolamine (PE, representative of the internal monolayer). Venous blood was drawn from patient with CRF before and after the HD procedure. Lipids from the erythrocyte stroma were extracted using the Rose and Oklander method and then were separated into phospholipid fractions using thin-layer chromatography (TLC). PE and SPH fractions were extracted, and the fatty acid profile was determined using gas chromatography (Perkin Elmer 8400; RTx 2330 column; length: 105 m). In the phospholipid fractions tested, a high content of saturated FA with a medium carbon chain (C 16:0 to C 18:2) and a long carbon chain such as C 24:0, C 24:1; C 22:6; and C 26:0 was found. The HD procedure affected the FA profile in the fractions tested. The proportion of saturated and unsaturated long-chain FA (above 18 C) increased in PE. However, the content of medium-chain FA C 16:0 to C 18:1 decreased. A significant decrease in the content of the majority of long-chain FA could be noted in SPH. The ratio of unsaturated (U) to saturated (S) fatty acids in the SPH fraction increased. Hemodialysis has a significant effect on the content of fatty acids in the PE and SPH fractions of erythrocyte membranes in patients with CRF. |
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Authors:
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Barbara Dolegowska; Ewa Kwiatkowska; Teresa Wesolowska; Joanna Bober; Dariusz Chlubek; Kazimierz Ciechanowski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Renal failure Volume: 29 ISSN: 0886-022X ISO Abbreviation: Ren Fail Publication Date: 2007 |
Date Detail:
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Created Date: 2007-05-14 Completed Date: 2007-08-14 Revised Date: 2008-05-21 |
Medline Journal Info:
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Nlm Unique ID: 8701128 Medline TA: Ren Fail Country: United States |
Other Details:
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Languages: eng Pagination: 447-52 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland. basiad@sci.pam.szczecin.pl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Thin Layer Erythrocyte Membrane / chemistry* Fatty Acids / metabolism* Humans Kidney Failure, Chronic / metabolism* Middle Aged Phosphatidylethanolamines / analysis Renal Dialysis* Sphingomyelins / analysis |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Phosphatidylethanolamines; 0/Sphingomyelins; 39382-08-6/phosphatidylethanolamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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