Document Detail

Effect of hemin on the synthesis of hemoglobin and other proteins in mammalian cells.
MedLine Citation:
PMID:  4197926     Owner:  NLM     Status:  MEDLINE    
The initiation of globin synthesis in intact reticulocytes and in reticulocyte lysates is maintained by the addition of hemin. The specificity of this effect has been studied to determine whether it is restricted to hemoglobin and erythroid cells. In intact reticulocytes, hemin (500 muM) enhances the synthesis of carbonic anhydrase as well as of hemoglobin. Similar enhancement of protein synthesis is observed on addition of hemin (500 muM) to intact Krebs II ascites tumor cells, in cell-free extracts of these cells, added hemin (50 muM) increases endogenous protein synthesis and the translation of exogenous rabbit globin messenger RNA. These results provide evidence that the effect of hemin is not restricted to globin, and they suggest that hemin may enhance protein synthesis in at least some nonerythroid cells.
Y Beuzard; R Rodvien; I M London
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  70     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1973 Apr 
Date Detail:
Created Date:  1973-09-12     Completed Date:  1973-09-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1022-6     Citation Subset:  IM    
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MeSH Terms
Alanine / metabolism
Carbon Isotopes
Carbonic Anhydrases / biosynthesis*,  isolation & purification
Carcinoma, Krebs 2
Cell Line
Chromatography, Ion Exchange
Electrophoresis, Polyacrylamide Gel
Globins / biosynthesis,  isolation & purification
Heme / pharmacology*
Hemoglobins / biosynthesis*,  isolation & purification
Neoplasm Proteins / biosynthesis
Protein Biosynthesis / drug effects
RNA, Messenger / metabolism
Reticulocytes / drug effects,  enzymology,  metabolism
Reg. No./Substance:
0/Carbon Isotopes; 0/Hemoglobins; 0/Neoplasm Proteins; 0/RNA, Messenger; 14875-96-8/Heme; 56-41-7/Alanine; 9004-22-2/Globins; EC Anhydrases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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