Document Detail


Effect of heat treatment on phage susceptibility of a Mycobacterium smegmatis strain.
MedLine Citation:
PMID:  3105227     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plating efficiency of mycobacteriophage butyricum (By) proved to be 10(-4)-10(-3) on Mycobacterium smegmatis strain Rabinowitz (M. sm. R.) cells being in the logarithmic phase. This increased to 10(-2)-10(-1) when the cells were held at 50-57 degrees C for 2 h before infection. After replacing the cells to 37 degrees C, plating efficiency of the phages returned to the starting values. This phenomenon could be inhibited by nalidixic acid (150 micrograms/ml) and chloramphenicol (10 micrograms/ml) but not by mitomycin C (0.05 microgram/ml). No return to the starting plating efficiency values were observed, if incubation of cells at 37 degrees C after heat treatment has been performed in buffer. The data suggest that By phage propagation in M. sm. R. cells is inhibited by a thermosensitive protein.
Authors:
K J Háber; I Földes
Related Documents :
22892127 - Ultra-violet b (uvb)-induced skin cell death occurs through a cyclophilin d intrinsic s...
2390227 - Effect of vitamin a on chemotactic and chemoinvasive behaviour of an osteosarcoma cell ...
22345767 - A comparison between different existing methods used to separate epidermal cells from s...
19353567 - Cytotoxic effects of different concentrations of a carbamide peroxide bleaching gel on ...
10335947 - Unusual choristoma of the parotid gland in a girl. a possible trichoadenoma.
9138477 - Comparison of the melanogenesis in human black and light brown melanocytes.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta microbiologica Hungarica     Volume:  33     ISSN:  0231-4622     ISO Abbreviation:  Acta Microbiol. Hung.     Publication Date:  1986  
Date Detail:
Created Date:  1987-05-01     Completed Date:  1987-05-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8400270     Medline TA:  Acta Microbiol Hung     Country:  HUNGARY    
Other Details:
Languages:  eng     Pagination:  257-62     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Chloramphenicol / pharmacology
Hot Temperature
Mitomycin
Mitomycins / pharmacology
Mycobacteriophages / drug effects,  growth & development*
Mycobacterium / physiology*
Nalidixic Acid / pharmacology
Plaque Assay
Chemical
Reg. No./Substance:
0/Mitomycins; 389-08-2/Nalidixic Acid; 50-07-7/Mitomycin; 56-75-7/Chloramphenicol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Immunochemical and immunological study of cell-wall proteins of Pseudomonas aeruginosa.
Next Document:  Selective localization of gamma-enolase in stromal cells of cerebellar hemangioblastomas.