Document Detail


Effect of halofenate and clofibrate on growth and lipid synthesis in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  4570609     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Halofenate-free acid (HFA) inhibited the growth of Saccharomyces cerevisiae by 50% at a concentration of 0.34 mm. This inhibitory effect was prevented by addition of either oleate or acetate, but not by pyruvate. When cell growth was supported by oleate, HFA inhibited the incorporation of radioactive carbon from glucose-U-(14)C or pyruvate-2-(14)C into fatty acids and sterols. The incorporation of radioactive carbon into fatty acids and sterols from acetate-2-(14)C was unaffected by the compound. When cell growth was supported by either oleate or acetate, HFA inhibited the conversion of pyruvate-1-(14)C to (14)CO(2). These results suggest that HFA inhibits the conversion of pyruvate to acetate in yeast. Partially purified yeast pyruvate dehydrogenase was inhibited 50% by 5.5 mm HFA; however, the concentration required for 50% inhibition was considerably reduced when the enzyme was preincubated with the compound at room temperature. In a similar manner, the hypolipidemic agent clofibrate-free acid inhibited the growth of yeast by 50% at 3.0 mm. This inhibition was also prevented by acetate and not by pyruvate. In addition, clofibrate-free acid inhibited partially purified pyruvate dehydrogenase by 50% at a concentration of 37.0 mm.
Authors:
M D Greenspan; J I Germershausen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  113     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1973 Feb 
Date Detail:
Created Date:  1973-05-03     Completed Date:  1973-05-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  847-55     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetates / metabolism
Antilipemic Agents / pharmacology*
Carbon Dioxide / biosynthesis
Carbon Isotopes
Chromatography, Gas
Clofibrate / pharmacology*
Fatty Acids / biosynthesis
Glucose / metabolism
Glycolates / pharmacology*
Halofenate / pharmacology
Lipids / biosynthesis*
Mass Spectrometry
Oleic Acids / metabolism
Pyruvate Oxidase / antagonists & inhibitors
Pyruvates / metabolism
Saccharomyces cerevisiae / drug effects*,  enzymology,  growth & development,  metabolism
Sterols / biosynthesis
Chemical
Reg. No./Substance:
0/Acetates; 0/Antilipemic Agents; 0/Carbon Isotopes; 0/Fatty Acids; 0/Glycolates; 0/Lipids; 0/Oleic Acids; 0/Pyruvates; 0/Sterols; 124-38-9/Carbon Dioxide; 26718-25-2/Halofenate; 50-99-7/Glucose; 637-07-0/Clofibrate; EC 1.2.3.3/Pyruvate Oxidase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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