Document Detail

Effect of growth factors on matrix synthesis by human nasal chondrocytes cultured in monolayer and in agar.
MedLine Citation:
PMID:  8858257     Owner:  NLM     Status:  MEDLINE    
Reconstructive surgery of multiple areas of the body may require replacement bone or cartilage transplants to repair defects or lesions of skeletal tissue. Advances in cell and tissue culture techniques now permit synthesis of autologous human cartilage in vitro. Several growth factors regulate the metabolism and activation of cartilage cells. To enhance culture conditions and effectiveness for in vitro cartilage engineering, the aim of our investigations was to characterize the influence of transforming growth factor (TGF)-beta and basic fibroblast growth factor (bFGF) on human nasal septal chondrocytes. The isolated cells were cultured as monolayers on plastic and in soft agar. The biological effects of the growth factors were assessed by determining synthesis of total protein and proteoglycan. TGF-beta caused a dose-dependent stimulation of total protein as well as glycosaminoglycan synthesis by all chondrocytes cultured. This stimulatory effect of TGF-beta was greater for chondrocytes cultured in soft agar than for chondrocytes cultured on plastic. No stimulatory effects of matrix synthesis was observed for bFGF in either culture condition. Our results show that TGF-beta can be employed to enhance in vitro production of cartilage grafts for reconstructive surgery.
J Bujía; P Pitzke; E Kastenbauer; E Wilmes; C Hammer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery     Volume:  253     ISSN:  0937-4477     ISO Abbreviation:  Eur Arch Otorhinolaryngol     Publication Date:  1996  
Date Detail:
Created Date:  1996-12-24     Completed Date:  1996-12-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9002937     Medline TA:  Eur Arch Otorhinolaryngol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  336-40     Citation Subset:  IM    
HNO-Klinik und Poliklinik, Klinikum Grosshadem, Munich, Germany.
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MeSH Terms
Cartilage / cytology*,  drug effects,  metabolism*
Cells, Cultured / drug effects
Culture Media
Culture Techniques / methods
Extracellular Matrix Proteins / biosynthesis*
Fibroblast Growth Factor 2 / pharmacology*
Glycosaminoglycans / biosynthesis
Nasal Septum
Proteoglycans / biosynthesis
Transforming Growth Factor beta / pharmacology*
Reg. No./Substance:
0/Culture Media; 0/Extracellular Matrix Proteins; 0/Glycosaminoglycans; 0/Proteoglycans; 0/Transforming Growth Factor beta; 103107-01-3/Fibroblast Growth Factor 2

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