Document Detail


Effect of gossypol on hepatic and serum gamma-glutamyltransferase activity in rats.
MedLine Citation:
PMID:  9232775     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A single intraperitoneal dose (25 mg/kg) of gossypol given to male Sprague-Dawley rats caused marked changes in the activity of the hepatic and serum gamma-glutamyltransferase (GGT) and microsomal monooxygenases. The GGT activity in liver homogenate, S-9 supernatant fraction and microsomes was significantly depressed; however, the level of serum GGT was elevated. While the hepatic glutathione concentration was not greatly changed, the aminopyrine N-demethylase activity and microsomal cytochrome P450 content of the liver were significantly decreased in the treated rats. At necropsy, the livers of the treated rats appeared generally pale with distinct pinpoint foci. Histopathological examination of the liver showed degenerative changes and coagulative necrosis. The results indicate that gossypol is a strong hepatotoxic agent which can produce severe hepatic damage.
Authors:
D P Deoras; P Young-Curtis; R R Dalvi; F E Tippett
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Veterinary research communications     Volume:  21     ISSN:  0165-7380     ISO Abbreviation:  Vet. Res. Commun.     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-09-11     Completed Date:  1997-09-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8100520     Medline TA:  Vet Res Commun     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  317-23     Citation Subset:  IM    
Affiliation:
Department of Physiology and Pharmacology, School of Veterinary Medicine, Tuskegee University, AL 36088, USA.
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MeSH Terms
Descriptor/Qualifier:
Aminopyrine N-Demethylase / drug effects,  metabolism
Animal Feed
Animals
Cytochrome P-450 Enzyme System / drug effects,  metabolism
Glutathione / drug effects,  metabolism
Gossypol / adverse effects,  pharmacology*
Infusions, Parenteral
Liver / enzymology*
Male
Microsomes, Liver / drug effects,  enzymology*
Necrosis
Rats
Rats, Sprague-Dawley
gamma-Glutamyltransferase / drug effects*,  metabolism
Grant Support
ID/Acronym/Agency:
1G12RRO3059/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
303-45-7/Gossypol; 70-18-8/Glutathione; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.5.3.-/Aminopyrine N-Demethylase; EC 2.3.2.2/gamma-Glutamyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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