| Effect of glycosylphosphatidylinositol specific phospholipase D gene expression levels on complement mediated killing of leukemic cells in patients with chronic myeloid leukemia. | |
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MedLine Citation:
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PMID: 15907827 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: To explore the disparity in glycosylphosphatidylinositol phospholipase D (GPI-PLD) expression levels between mononuclear cells of chronic myeloid leukemia (CML) and healthy controls, and clarify the certain relation of GPI-PLD expression levels to complement mediated killing of leukemic cells. METHODS: Competitive RT-PCR was used to detect quantitatively the GPI-PLD mRNA in mononuclear cells. GPI-anchored CD55 and CD59 were analyzed by flow cytometry and Western blotting. Complement-mediated lysis was assessed by staining method of trypan blue dye. RESULTS: The GPI-PLD activities and their mRNA copies in CML patients were significantly lower than those in healthy adults. At the tenth day after treatment with bone marrow transplantation (BMT), the GPI-PLD activities and copies of GPI-PLD mRNA almost recovered to the expression levels of healthy subjects. The expression of both CD55 and CD59 in CML patients were significantly higher than those in healthy subjects. After treatment with insulin (10(-7) mol/l) plus glucose (16.7 mmol/l) for 48 h, the cellular GPI-PLD activity and mRNA levels in K562 cells derived from the leukemic cells of a CML patient all increased about 3-fold. Simultaneously, the GPI-anchored CD55 and CD59 on cell surfaces were released into the culturing medium, and the killing rate of complement-mediated K562 cell lysis increased almost 3 times. CONCLUSION: The decreased GPI-PLD expression may reduce the release of GPI-anchored CD55 and CD59 in leukemia cells and finally decrease complement mediated killing of these cells in chronic phase of CML. |
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Authors:
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Tang Jian-Hua; Zhang Xiao-Jie; Wang Yi-Dan; Li Wen-Kai; He Wang-Jiao; Wang Cheng-Hong; Li Gui-Yuan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 359 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 2005 Sep |
Date Detail:
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Created Date: 2005-08-09 Completed Date: 2005-11-07 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 115-24 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Institute of Biological Science and Technology, Central South University, Xiangya Road 88, Mail box 54number, Changsha, Hunan 410078, PR China. miket561@xysm.net |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antigens, CD55 / metabolism Antigens, CD59 / metabolism Base Sequence Blotting, Western Case-Control Studies Cell Death / physiology* Complement System Proteins / physiology* DNA Primers Flow Cytometry Gene Expression* Glycosylphosphatidylinositols / metabolism* Humans K562 Cells Leukemia, Myelogenous, Chronic, BCR-ABL Positive / enzymology, pathology* Phospholipase D / genetics*, metabolism RNA, Messenger / genetics Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD55; 0/Antigens, CD59; 0/DNA Primers; 0/Glycosylphosphatidylinositols; 0/RNA, Messenger; 9007-36-7/Complement System Proteins; EC 3.1.4.4/Phospholipase D |
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