Document Detail

Effect of glycosylphosphatidylinositol specific phospholipase D gene expression levels on complement mediated killing of leukemic cells in patients with chronic myeloid leukemia.
MedLine Citation:
PMID:  15907827     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: To explore the disparity in glycosylphosphatidylinositol phospholipase D (GPI-PLD) expression levels between mononuclear cells of chronic myeloid leukemia (CML) and healthy controls, and clarify the certain relation of GPI-PLD expression levels to complement mediated killing of leukemic cells. METHODS: Competitive RT-PCR was used to detect quantitatively the GPI-PLD mRNA in mononuclear cells. GPI-anchored CD55 and CD59 were analyzed by flow cytometry and Western blotting. Complement-mediated lysis was assessed by staining method of trypan blue dye. RESULTS: The GPI-PLD activities and their mRNA copies in CML patients were significantly lower than those in healthy adults. At the tenth day after treatment with bone marrow transplantation (BMT), the GPI-PLD activities and copies of GPI-PLD mRNA almost recovered to the expression levels of healthy subjects. The expression of both CD55 and CD59 in CML patients were significantly higher than those in healthy subjects. After treatment with insulin (10(-7) mol/l) plus glucose (16.7 mmol/l) for 48 h, the cellular GPI-PLD activity and mRNA levels in K562 cells derived from the leukemic cells of a CML patient all increased about 3-fold. Simultaneously, the GPI-anchored CD55 and CD59 on cell surfaces were released into the culturing medium, and the killing rate of complement-mediated K562 cell lysis increased almost 3 times. CONCLUSION: The decreased GPI-PLD expression may reduce the release of GPI-anchored CD55 and CD59 in leukemia cells and finally decrease complement mediated killing of these cells in chronic phase of CML.
Tang Jian-Hua; Zhang Xiao-Jie; Wang Yi-Dan; Li Wen-Kai; He Wang-Jiao; Wang Cheng-Hong; Li Gui-Yuan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  359     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-08-09     Completed Date:  2005-11-07     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  115-24     Citation Subset:  IM    
Department of Biochemistry, Institute of Biological Science and Technology, Central South University, Xiangya Road 88, Mail box 54number, Changsha, Hunan 410078, PR China.
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MeSH Terms
Antigens, CD55 / metabolism
Antigens, CD59 / metabolism
Base Sequence
Blotting, Western
Case-Control Studies
Cell Death / physiology*
Complement System Proteins / physiology*
DNA Primers
Flow Cytometry
Gene Expression*
Glycosylphosphatidylinositols / metabolism*
K562 Cells
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / enzymology,  pathology*
Phospholipase D / genetics*,  metabolism
RNA, Messenger / genetics
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Antigens, CD55; 0/Antigens, CD59; 0/DNA Primers; 0/Glycosylphosphatidylinositols; 0/RNA, Messenger; 9007-36-7/Complement System Proteins; EC D

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