Document Detail

Effect of glycine and leucine on the recovery of frozen-thawed Lavandula vera cells.
MedLine Citation:
PMID:  12148046     Owner:  NLM     Status:  Publisher    
Ammonium ion is known to inhibit the recovery of frozen-thawed Lavendula vera cells from freezing injury. In this study, we examined the effect of glycine and leucine on the recovery of frozen-thawed L. vera cells. Both amino acids show a more deleterious effect on the recovery of freeze-injured cells than ammonium ion according to the TTC assay of the post-thaw cultured cells. However, the deleterious effect differed in quality between glycine and leucine. Glycine not only inhibited the recovery of frozen-thawed cells but also promoted the lethal process of freezing injury. Although leucine did not promote the lethal process of freezing-injury, it did inhibit the recovery of freezing injury.
A Kuriyama; K Watanabe; M Yamana
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Publication Detail:
Journal Detail:
Title:  Cryo letters     Volume:  21     ISSN:  0143-2044     ISO Abbreviation:  Cryo Letters     Publication Date:    2000 May-Jun
Date Detail:
Created Date:  2002-Jul-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9891832     Medline TA:  Cryo Letters     Country:  -    
Other Details:
Languages:  ENG     Pagination:  157-162     Citation Subset:  -    
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