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Effect of glucose concentration during embryoid body (EB) formation from mouse embryonic stem cells on EB growth and cell differentiation.
MedLine Citation:
PMID:  20869914     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Embryoid body (EB) formation is an important step in the differentiation of pluripotent stem cells. Although glucose concentration is physiologically maintained at 5.5mM (low glucose; LG) in vivo, a medium containing 25 mM glucose (high glucose; HG) has been widely used for forming EBs in vitro. In this study, we investigated the effect of glucose concentration during EB formation from mouse embryonic stem (ES) cells on EB growth and cell differentiation. EBs were formed under various glucose concentrations: 40, 25, 5.5, and 0mM. Cells aggregated to form EBs regardless of glucose concentration, but 0mM glucose was not appropriate for supporting EB growth as compared with 25 mM glucose. The EBs that formed in the presence of 5.5mM glucose (LG-EBs) were similar both in terms of appearance and decreased expression levels of undifferentiated-ES-cell-marker genes to the EBs that formed in the presence of 25 mM glucose (HG-EBs). However, there was a difference in the propensity for cell differentiation between LG-EBs and HG-EBs. In directed differentiation cultures of EBs into cardiomyocytes and neuronal cells, the HG-EBs more efficiently generated beating cardiac muscle, and the LG-EBs more specifically generated βIII-tubulin-positive cells. These findings demonstrate that the high-glucose (25 mM) condition was not necessary for EB formation in mouse ES cells, whereas the glucose concentration during EB formation affects the propensity for cell differentiation in the attachment cultures of formed EBs. The physiological low-glucose (5.5mM) condition was suitable for forming EBs directed toward neuronal cell differentiation in mouse ES cells.
Authors:
Hidemi Mochizuki; Yoshitsugu Ohnuki; Hiroshi Kurosawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-24
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  111     ISSN:  1347-4421     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  92-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
Affiliation:
Division of Medicine and Engineering Science, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan.
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