Document Detail


Effect of glucose on the fatty acid composition of Cupriavidus necator JMP134 during 2,4-dichlorophenoxyacetic acid degradation: implications for lipid-based stable isotope probing methods.
MedLine Citation:
PMID:  21856833     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Combining lipid biomarker profiling with stable isotope probing (SIP) is a powerful technique for studying specific microbial populations responsible for the degradation of organic pollutants in various natural environments. However, the presence of other easily degradable substrates may induce significant physiological changes by altering both the rate of incorporation of the target compound into the biomass and the microbial lipid profiles. In order to test this hypothesis, Cupriavidus necator JMP134, a 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacterium, was incubated with [(13)C]2,4-D, [(13)C]glucose, or mixtures of both substrates alternatively labeled with (13)C. C. necator JMP134 exhibited a preferential use of 2,4-D over glucose. The isotopic analysis showed that glucose had only a small effect on the incorporation of the acetic chain of 2,4-D into the biomass (at days 2 and 3) and no effect on that of the benzenic ring. The addition of glucose did change the fatty acid methyl ester (FAME) composition. However, the overall FAME isotopic signature reflected that of the entire biomass. Compound-specific individual isotopic analyses of FAME composition showed that the (13)C-enriched FAME profiles were slightly or not affected when tracing the 2,4-D acetic chain or 2,4-D benzenic ring, respectively. This batch study is a necessary step for validating the use of lipid-based SIP methods in complex environments.
Authors:
Thomas Z Lerch; Marie-France Dignac; Enrique Barriuso; André Mariotti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-08-19
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  77     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-06     Completed Date:  2012-02-02     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7296-306     Citation Subset:  IM    
Affiliation:
UMR Biogeochimie et Ecologie des Milieux Continentaux, CNRS-UMPC-ENS-AgroParisTech-IRD-UPEC, Campus INRA, 78850 Thiverval-Grignon, France. thomas.lerch@u-pec.fr
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MeSH Terms
Descriptor/Qualifier:
2,4-Dichlorophenoxyacetic Acid / metabolism*
Biotransformation
Carbon Isotopes / metabolism
Cupriavidus necator / chemistry*,  growth & development,  metabolism*
Environmental Microbiology
Fatty Acids / analysis*
Glucose / metabolism*
Isotope Labeling / methods
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Fatty Acids; 50-99-7/Glucose; 94-75-7/2,4-Dichlorophenoxyacetic Acid
Comments/Corrections

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