| Effect of genotype, gelling agent, and auxin on the induction of somatic embryogenesis in sweet potato (Ipomoea batatas Lam.). | |
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MedLine Citation:
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PMID: 18280985 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lateral buds of six cultivars of sweet potato were induced to form embryogenic callus in a culture medium solidified with two types of gelling agents, Agar or Gelrite, and supplemented with various concentrations of auxins, 2,4-D, 2,4,5-T and Picloram. Of the six cultivars screened, only three gave an embryogenic response. Best results with an average of 3.53% embryogenic response were obtained with the medium solidified with Agar, while in Gelrite only 0.45% of lateral buds gave rise to embryogenic callus. The interaction between the genotype and auxins was highly significant; particularly the optimal response was obtained with cv. Zho and 865 yielding 10.7 and 14.7% somatic embryogenesis, respectively, in the medium containing 2,4,5-T or Picloram. The plant conversion was dramatically improved by subculture of the embryogenic callus on the medium with the combination of 1 microM 2,4-D and 1 microM Kinetin or 5 microM ABA alone before transfer of mature embryos onto hormone-free medium. The embryogenic callus of sweet potato and its sustained ability to further regenerate plants have regularly been maintained for several years by frequent subculture in 5 microM 2,4,5-T or the combination of 10 microM 2,4-D and 1 microM BAP or kinetin. The embryo-derived plants seemed apparently genetically stable and similar to the hexaploid parental plants, based on morphological analysis and their ploidy level determined by using flow cytometry. |
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Authors:
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Zine El Abidine Triqui; Abdelkarim Guédira; Averil Chlyah; Hassane Chlyah; Vongthip Souvannavong; Robert Haïcour; Darasinh Sihachakr |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-21 |
Journal Detail:
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Title: Comptes rendus biologies Volume: 331 ISSN: 1631-0691 ISO Abbreviation: C. R. Biol. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-02-18 Completed Date: 2008-04-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101140040 Medline TA: C R Biol Country: France |
Other Details:
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Languages: eng Pagination: 198-205 Citation Subset: IM |
Affiliation:
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Laboratoire de physiologie végétale, département de biologie, faculté des sciences, BP 1014, Rabat, Maroc. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Agar
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pharmacology* Culture Media Embryonic Development Genotype* Indoleacetic Acids / pharmacology* Ipomoea batatas / embryology*, genetics* Ploidies Polysaccharides, Bacterial / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Indoleacetic Acids; 0/Polysaccharides, Bacterial; 71010-52-1/gellan gum; 9002-18-0/Agar |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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