Document Detail


Effect of genotype, gelling agent, and auxin on the induction of somatic embryogenesis in sweet potato (Ipomoea batatas Lam.).
MedLine Citation:
PMID:  18280985     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lateral buds of six cultivars of sweet potato were induced to form embryogenic callus in a culture medium solidified with two types of gelling agents, Agar or Gelrite, and supplemented with various concentrations of auxins, 2,4-D, 2,4,5-T and Picloram. Of the six cultivars screened, only three gave an embryogenic response. Best results with an average of 3.53% embryogenic response were obtained with the medium solidified with Agar, while in Gelrite only 0.45% of lateral buds gave rise to embryogenic callus. The interaction between the genotype and auxins was highly significant; particularly the optimal response was obtained with cv. Zho and 865 yielding 10.7 and 14.7% somatic embryogenesis, respectively, in the medium containing 2,4,5-T or Picloram. The plant conversion was dramatically improved by subculture of the embryogenic callus on the medium with the combination of 1 microM 2,4-D and 1 microM Kinetin or 5 microM ABA alone before transfer of mature embryos onto hormone-free medium. The embryogenic callus of sweet potato and its sustained ability to further regenerate plants have regularly been maintained for several years by frequent subculture in 5 microM 2,4,5-T or the combination of 10 microM 2,4-D and 1 microM BAP or kinetin. The embryo-derived plants seemed apparently genetically stable and similar to the hexaploid parental plants, based on morphological analysis and their ploidy level determined by using flow cytometry.
Authors:
Zine El Abidine Triqui; Abdelkarim Guédira; Averil Chlyah; Hassane Chlyah; Vongthip Souvannavong; Robert Haïcour; Darasinh Sihachakr
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-21
Journal Detail:
Title:  Comptes rendus biologies     Volume:  331     ISSN:  1631-0691     ISO Abbreviation:  C. R. Biol.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-18     Completed Date:  2008-04-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101140040     Medline TA:  C R Biol     Country:  France    
Other Details:
Languages:  eng     Pagination:  198-205     Citation Subset:  IM    
Affiliation:
Laboratoire de physiologie végétale, département de biologie, faculté des sciences, BP 1014, Rabat, Maroc.
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MeSH Terms
Descriptor/Qualifier:
Agar / pharmacology*
Culture Media
Embryonic Development
Genotype*
Indoleacetic Acids / pharmacology*
Ipomoea batatas / embryology*,  genetics*
Ploidies
Polysaccharides, Bacterial / pharmacology*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Indoleacetic Acids; 0/Polysaccharides, Bacterial; 71010-52-1/gellan gum; 9002-18-0/Agar

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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