Document Detail

Effect of genetic background on establishment of mouse embryonic stem cells.
MedLine Citation:
PMID:  10480028     Owner:  NLM     Status:  MEDLINE    
We established 13 embryonic stem (ES) cell lines from 542 embryos crossed between various strains of mice: 10 lines from 129/Sv-ter embryos (10/48, 20.8%) and 3 lines out of other 9 combinations of intra- or inter-strain matings (1 from intracross of C57BL/6CrSlc, 1 from B6D2F1 x C57BL/6CrSlc, 1 from Yok:ddY x Slc:ICR). No ES cell line from 129/Sv-ter x Slc:ICR embryos suggests that ICR strain might have inhibitory genetic factor(s) for the ES cell formation. Some ES cell lines could be obtained from hybrids even if none or few lines from their parental strains, suggesting a heterosis effect can be expected for establishing ES cell lines in mice.
O Suzuki; J Matsuda; K Takano; Y Yamamoto; T Asano; M Naiki; M Kusanagi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental animals / Japanese Association for Laboratory Animal Science     Volume:  48     ISSN:  1341-1357     ISO Abbreviation:  Exp. Anim.     Publication Date:  1999 Jul 
Date Detail:
Created Date:  1999-09-23     Completed Date:  1999-09-23     Revised Date:  2014-10-24    
Medline Journal Info:
Nlm Unique ID:  9604830     Medline TA:  Exp Anim     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  213-6     Citation Subset:  IM    
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MeSH Terms
Cell Line / physiology
Culture Techniques
Embryo, Mammalian
Mice, Inbred Strains / genetics*
Mice, Transgenic
Species Specificity
Stem Cells / physiology*

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