Document Detail


Effect of the gene transformer of Anastrepha on the somatic sexual development of Drosophila.
MedLine Citation:
PMID:  20209435     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The gene transformer (tra) is the key regulatory memory device for sex determination in tephritid insects. The present manuscript addressed the question about the functional conservation of the tephritid Anastrepha Transformer protein to direct somatic sexual development in Drosophila (Drosophilidae). The transformer cDNA of Anastrepha encoding the putative full-length Tra protein was cloned in pUAST and introduced into Drosophila melanogaster. To express this protein, the GAL4-UAS system was used. The Anastrepha Tra protein induced the female-specific splicing of both dsx and fru pre-mRNAs in Drosophila XY male flies, so that these became transformed into females, though this transformation was incomplete (the sexually dimorphic foreleg basitarsus and the external terminalia were monitored). It was found that the degree of female transformation directly depended on the dose of Anastrepha tra and Drosophila transformer-2 (tra-2) genes, and that the Anastrepha Tra-Drosophila Tra2 complex is not as efficient as the Drosophila Tra-Tra2 complex at inducing the female-specific splicing of Drosophila dsx pre-mRNA. This can explain why the Anastrepha Tra protein cannot fully substitute for the endogenous Drosophila Tra protein.
Authors:
María-Fernanda Ruiz; Lucas Sánchez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The International journal of developmental biology     Volume:  54     ISSN:  1696-3547     ISO Abbreviation:  Int. J. Dev. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-03-08     Completed Date:  2010-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8917470     Medline TA:  Int J Dev Biol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  627-33     Citation Subset:  IM    
Affiliation:
Centro de Investigaciones Biologicas (CSIC), Madrid, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
DNA, Complementary / genetics
Drosophila / genetics*
Female
Gene Expression Regulation
Male
Neurons / physiology*
Nuclear Proteins / genetics*
RNA Precursors / genetics
RNA Splicing
Sexual Development / genetics*
Tephritidae / genetics*
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Nuclear Proteins; 0/RNA Precursors; 0/transformer protein, Drosophila

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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