Document Detail

Effect of gelatin on the cyclic AMP response of primocultured hog thyroid cells to acute thyrotropin stimulation.
MedLine Citation:
PMID:  228749     Owner:  NLM     Status:  MEDLINE    
The cyclic AMP response of cultured hog thyroid cells to acute thyrotropin stimulation was shown to be under a dual regulatory control by thyrotropin: both positive and negative regulation have been described. When added to the culture medium, gelatin (0.25%) promoted the reorganization of the cells into folicle-like structures, as does thyrotropin. Unlike thyrotropin, gelatin did not induce an increase in intracellular cyclic AMP but enhanced the acute cyclic AMP response to thyrotropin in cells cultured in gelatin-containing medium. When both gelatin and thyrotropin were present, the positive effect of low concentrations of hormone (less than 50 microU/ml) was increased whereas the refractory process observed in the presence of higher concentrations of hormone (greater than 50 microU/ml) was unchanged. These effects of gelatin might be mediated by interaction of the denatured collagen molecules with external proteins of the plasma membrane of thyroid cells.
N Takasu; B Charrier; J Mauchamp; S Lissitzky
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  587     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1979 Nov 
Date Detail:
Created Date:  1980-02-15     Completed Date:  1980-02-15     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  507-14     Citation Subset:  IM    
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MeSH Terms
Bucladesine / pharmacology
Cells, Cultured
Cyclic AMP / metabolism*
Gelatin / pharmacology*
Prostaglandins E / pharmacology
Stimulation, Chemical
Thyroid Gland / metabolism*
Thyrotropin / metabolism,  pharmacology*
Reg. No./Substance:
0/Prostaglandins E; 362-74-3/Bucladesine; 60-92-4/Cyclic AMP; 9000-70-8/Gelatin; 9002-71-5/Thyrotropin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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