Document Detail

Effect of gap junctional intercellular communication on radiation responses in neoplastic human cells.
MedLine Citation:
PMID:  17316077     Owner:  NLM     Status:  MEDLINE    
Gap junctional intercellular communication (GJIC) is an important function of metazoan cells and is believed to have beneficial effects in anti-tumor therapy. In this study, we found that, when neoplastic human salivary gland (HSG) cells were irradiated with a 100 keV/microm carbon-ion beam, micronuclei, G(2)/M-phase arrest, and cell killing were induced and that their induction increased with dose. Treatment of confluent HSG cells with 8-Br-cAMP increased GJIC between cells. After release from this treatment, the cell cycle progress and the formation of binucleated cells were still similar to those of untreated cells. However, radiation-induced cellular damage, including micronucleus (MN) formation and G(2)/M-phase arrest of that cAMP-treated population, was less than that of the untreated population and that the surviving fraction was slightly enhanced by cAMP treatment, suggesting that increased GJIC protects HSG cells from lethal radiation damage. Moreover, when confluent HSG cells were treated with 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (PTIO), a scavenger of nitric oxide (NO) free radical, MN induction and cell killing in the irradiated population were increased. Our results indicate that NO may be involved in GJIC-mediated radioprotection of HSG cells, which may have implications for radiotherapy.
Chunlin Shao; Yoshiya Furusawa; Yoshitaka Matsumoto; Yan Pan; Ping Xu; Honghong Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Radiation research     Volume:  167     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-23     Completed Date:  2007-04-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  283-8     Citation Subset:  IM; S    
Institute of Radiation Medicine, Fudan University, Shanghai 200032, China.
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MeSH Terms
Cell Communication / drug effects,  radiation effects*
Cell Cycle / radiation effects
Cell Line, Tumor
Cell Nucleus / drug effects
Cyclic AMP / pharmacology
Cyclic N-Oxides / pharmacology
Gap Junctions / drug effects,  metabolism*,  radiation effects*
Imidazoles / pharmacology
Salivary Gland Neoplasms / metabolism*,  pathology*,  radiotherapy
Reg. No./Substance:
0/Cyclic N-Oxides; 0/Imidazoles; 18390-00-6/2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide; 60-92-4/Cyclic AMP

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