| Effect of gamma-interferon on the expression of major histocompatibility complex class I and II gene products in neural cells isolated from the developing human fetal peripheral nervous system. | |
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MedLine Citation:
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PMID: 1380249 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of gamma-interferon (gamma-IFN) on the expression of major histocompatibility complex (MHC) gene products was examined in the developing human fetal peripheral nervous system. RNA blot hybridization analysis of total RNA isolated from human fetal dorsal root ganglia (DRG) neural cell populations cultured in vitro for 5 days resulted in the detection of both MHC class I- and class II-specific RNAs. As determined by protein immunoblotting and fluorescence-activated flow cytometry, MHC class I and II proteins were also readily detectable in cultured human fetal DRG neural cell populations 5 days after isolation. In addition, treatment of 3-day human fetal DRG neural cells with gamma-IFN (100 U/ml; 48 h) resulted in a marked increase in the level of MHC class I- and class II-specific RNA and protein without inhibiting the proliferation of the neural cell population. These results suggest that changes in the levels of selected cytokines such as gamma-IFN may alter the ability of specific neural cell populations present in the developing human nervous system to participate in immune reactions by alteration of MHC class I and II antigen expression which may lead to perturbation in glial cell function and ultimately to nervous system dysfunction in general. |
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Authors:
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B B Kollas; H T Hartle; B Wigdahl |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Fetal diagnosis and therapy Volume: 7 ISSN: 1015-3837 ISO Abbreviation: Fetal. Diagn. Ther. Publication Date: 1992 |
Date Detail:
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Created Date: 1992-09-22 Completed Date: 1992-09-22 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9107463 Medline TA: Fetal Diagn Ther Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 62-74 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, Pennsylvania State University, College of Medicine, Hershey 17033. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Division Fetus / cytology, immunology* Ganglia, Spinal / cytology, immunology Gene Expression Histocompatibility Antigens Class I / biosynthesis, genetics Histocompatibility Antigens Class II / biosynthesis, genetics Humans Interferon-gamma / pharmacology* Neurons / cytology, immunology Peripheral Nerves / cytology, immunology* RNA / genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AI 24484/AI/NIAID NIH HHS; NS 27405/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Histocompatibility Antigens Class I; 0/Histocompatibility Antigens Class II; 63231-63-0/RNA; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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