| Effect of gamma-glutamylcysteine ethylester on the levels of c-fos mRNA expression, glutathione and reactive oxygen species formation in kainic acid excitotoxicity. | |
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MedLine Citation:
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PMID: 20663035 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: The aim of this study was to investigate the effect of gamma-glutamylcysteine ethylester (GCEE), a precursor of glutathione biosynthesis, on the levels of glutathione, formation of reactive oxygen species and c-fos mRNA expression in rat hippocampus and cortex in kainic acid-induced excitotoxicity. METHODS: Sprague-Dawley rats were used and divided into four groups: control, kainic acid (10 mg/kg), GCEE (10 mg/kg) and kainic acid (10 mg/kg) + GCEE (10 mg/kg). Kainic acid and GCEE were administered to the rats intraperitoneally. The levels of glutathione and the expressions of c-fos mRNA in hippocampus and cortex tissues were determined using spectrophotometric and reverse transcription followed real-time PCR methods, respectively. Formation of reactive oxygen species was determined using dichlorofluorescin fluorescence in brain synaptosomes treated with kainic acid or GCEE in vitro. KEY FINDINGS: Kainic acid treatment significiantly upregulated the expression of c-fos mRNA in the hippocampus and cortex when compared to the control group. GCEE treatment significantly decreased the levels of c-fos mRNA in the cortex when compared to the kainic acid-treated group. GCEE treatment against kainic acid significantly increased the levels of glutathione in the cortex and hippocampus, and decreased the levels of formation of reactive oxygen species when compared to kainic acid-treated synaptosomes. CONCLUSIONS: The increased levels of glutathione and the reduced levels of reactive oxygen species formation lead us to conclude that GCEE may be beneficial as a potential antioxidant against neurodegenerative processes where excitotoxicity is involved. |
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Authors:
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Ezgi Turunc; Lutfiye Kanit; Ayfer Yalcin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of pharmacy and pharmacology Volume: 62 ISSN: 2042-7158 ISO Abbreviation: J. Pharm. Pharmacol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-28 Completed Date: 2010-11-15 Revised Date: 2011-09-13 |
Medline Journal Info:
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Nlm Unique ID: 0376363 Medline TA: J Pharm Pharmacol Country: England |
Other Details:
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Languages: eng Pagination: 1010-7 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Faculty of Pharmacy, Faculty of Medicine, Ege University, Izmir, Turkey. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antioxidants / administration & dosage, pharmacology* Cerebral Cortex / drug effects*, metabolism Dipeptides / administration & dosage, pharmacology* Epilepsy, Tonic-Clonic / chemically induced Genes, fos* Glutathione / metabolism* Hippocampus / drug effects*, metabolism Injections, Intraperitoneal Kainic Acid / administration & dosage, toxicity* Male Motor Activity / drug effects RNA, Messenger / metabolism* Rats Rats, Sprague-Dawley Reactive Oxygen Species / metabolism* Reverse Transcriptase Polymerase Chain Reaction Spectrophotometry Synaptosomes / drug effects, metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Dipeptides; 0/RNA, Messenger; 0/Reactive Oxygen Species; 114627-30-4/N-gamma-glutamylcysteine ethyl ester; 487-79-6/Kainic Acid; 70-18-8/Glutathione |
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