| Effect of gallic acid on alkaline phosphatase and peptidase activities in rat intestine. | |
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MedLine Citation:
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PMID: 20027867 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Gallic acid is a normal constituent of many edible foods, thus directly interacts with epithelial tissue in intestine. In the present study, the effect of gallic acid on intestinal alkaline phosphatase (IAP) and peptidase activities in rat intestine was evaluated. Gallic acid (0.27-0.5 mM) inhibited activities of leucine aminopeptidase (LAP) and y-glutamyl transpeptidase (y-GTP) by over 90%, compared to controls in rat intestine. In contrast, 0.1-0.6 mM gallic acid either had no effect or stimulated the activity of IAP in rat intestine. The observed inhibition of peptidases by gallic acid was reversible in nature. Kinetic analysis revealed no change in Vmx of LAP (0.42-0.44 units/mg protein) and gamma-GTP (0.22-0.24 units/mg protein), while the values of apparent Km were increased 6-7 fold, exhibiting competitive-type of enzyme inhibition by gallic acid. The values of Ki for LAP and gamma-GTP were 0.037 mM and 0.017 mM, respectively. These observations indicate that gallic acid is a potent inhibitor of brush border peptidases, and thus may interfere in the digestion and absorption of proteins in the intestine. |
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Authors:
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Nidhi Mahajan; Akhtar Mahmood |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Indian journal of biochemistry & biophysics Volume: 46 ISSN: 0301-1208 ISO Abbreviation: Indian J. Biochem. Biophys. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-12-23 Completed Date: 2010-09-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0310774 Medline TA: Indian J Biochem Biophys Country: India |
Other Details:
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Languages: eng Pagination: 378-82 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Panjab University, Chandigarh, 160014, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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antagonists & inhibitors,
metabolism* Animals Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology Gallic Acid / pharmacology* Intestines / drug effects*, enzymology*, metabolism Kinetics Leucyl Aminopeptidase / antagonists & inhibitors, metabolism* Male Rats Rats, Wistar gamma-Glutamyltransferase / antagonists & inhibitors, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 149-91-7/Gallic Acid; EC 2.3.2.2/gamma-Glutamyltransferase; EC 3.1.3.1/Alkaline Phosphatase; EC 3.4.11.1/Leucyl Aminopeptidase |
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