Document Detail


Effect of gallic acid on alkaline phosphatase and peptidase activities in rat intestine.
MedLine Citation:
PMID:  20027867     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Gallic acid is a normal constituent of many edible foods, thus directly interacts with epithelial tissue in intestine. In the present study, the effect of gallic acid on intestinal alkaline phosphatase (IAP) and peptidase activities in rat intestine was evaluated. Gallic acid (0.27-0.5 mM) inhibited activities of leucine aminopeptidase (LAP) and y-glutamyl transpeptidase (y-GTP) by over 90%, compared to controls in rat intestine. In contrast, 0.1-0.6 mM gallic acid either had no effect or stimulated the activity of IAP in rat intestine. The observed inhibition of peptidases by gallic acid was reversible in nature. Kinetic analysis revealed no change in Vmx of LAP (0.42-0.44 units/mg protein) and gamma-GTP (0.22-0.24 units/mg protein), while the values of apparent Km were increased 6-7 fold, exhibiting competitive-type of enzyme inhibition by gallic acid. The values of Ki for LAP and gamma-GTP were 0.037 mM and 0.017 mM, respectively. These observations indicate that gallic acid is a potent inhibitor of brush border peptidases, and thus may interfere in the digestion and absorption of proteins in the intestine.
Authors:
Nidhi Mahajan; Akhtar Mahmood
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Indian journal of biochemistry & biophysics     Volume:  46     ISSN:  0301-1208     ISO Abbreviation:  Indian J. Biochem. Biophys.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-12-23     Completed Date:  2010-09-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0310774     Medline TA:  Indian J Biochem Biophys     Country:  India    
Other Details:
Languages:  eng     Pagination:  378-82     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Panjab University, Chandigarh, 160014, India.
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / antagonists & inhibitors,  metabolism*
Animals
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology
Gallic Acid / pharmacology*
Intestines / drug effects*,  enzymology*,  metabolism
Kinetics
Leucyl Aminopeptidase / antagonists & inhibitors,  metabolism*
Male
Rats
Rats, Wistar
gamma-Glutamyltransferase / antagonists & inhibitors,  metabolism*
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 149-91-7/Gallic Acid; EC 2.3.2.2/gamma-Glutamyltransferase; EC 3.1.3.1/Alkaline Phosphatase; EC 3.4.11.1/Leucyl Aminopeptidase

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