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Effect of furanone on experimentally induced Pseudomonas aeruginosa biofilm formation: In vitro study.
MedLine Citation:
PMID:  22884365     Owner:  NLM     Status:  Publisher    
OBJECTIVE: The purpose of this study was to evaluate the effect of furanone as a quorum sensing inhibitor with an antibiotic agent on experimentally induced Pseudomonas aeruginosa biofilm formation in an in vitro model. MATERIALS AND METHODS: Bacterial cultures on agar plates and induction of biofilm formations were done in four different conditions; P. aeruginosa only (group 1), P. aeruginosa+ciprofloxacin (group 2), P. aeruginosa+furanone (group 3), and P. aeruginosa+furanone+ciprofloxacin (group 4). After biofilm formation on a silicone tympanostomy tube with P. aeruginosa was established, they were treated with ciprofloxacin, furanone, and ciprofloxacin with furanone respectively. All agar plates were processed for colony counts 1 day after incubation, and silicone tympanostomy tubes were observed using scanning electron microscopy 2 days after incubation. Any changes visualized on the biofilm lining the silicone tympanostomy tubes after treatment initiation were evaluated again 2 days after each treatment. RESULTS: There was no bacterial growth on the agar plate in group 4 compared to other groups and the formations of a biofilm on the surface of silicone tympanostomy tubes in group 4 were devoid. Little change to the original biofilm overlying the silicone tympanostomy tubes after each treatment was noted. CONCLUSION: This study demonstrates that furanone may have a role as a QSI and can be helpful in preventing biofilm formation with ciprofloxacin in experimentally induced P. aeruginosa biofilms based on an in vitro model.
Sun Gui Kim; Young Hoon Yoon; Jin Woong Choi; Ki Sang Rha; Yong Ho Park
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-7
Journal Detail:
Title:  International journal of pediatric otorhinolaryngology     Volume:  -     ISSN:  1872-8464     ISO Abbreviation:  Int. J. Pediatr. Otorhinolaryngol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003603     Medline TA:  Int J Pediatr Otorhinolaryngol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
Department of Otolaryngology-Head and Neck Surgery, College of Medicine Chungnam National University, Daejeon, Republic of Korea.
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