Document Detail


Effect of fructose and other carbohydrates on the surface properties, lipoteichoic acid production, and extracellular proteins of Streptococcus mutans Ingbritt grown in continuous culture.
MedLine Citation:
PMID:  7216459     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Streptococcus mutans Ingbritt was grown in a chemostat at destined dilution rates in either 0.5% fructose or 0.5% sorbitol and at destined pH values in 0.5% fructose. The yield of cells was affected by the carbohydrate source, as well as by the pH, with the lowest yield being at pH 5.5 in 0.5% fructose. Fructose-grown cells showed greater susceptibility to lysis by a muramidase than the corresponding glucose-grown cells, but there were no marked differences in the lytic susceptibilities of the corresponding cell wall preparations or in the serological reactivities of wall lysates with antiserum to S. mutans Ingbritt. The greatest amounts of cellular lipoteichoic acid were obtained at high dilution rates in both fructose and sorbitol, as well as at high pH values in fructose. The greatest amounts of extracellular lipoteichoic acid were found at low dilution rates, as estimated by rocket immunoelectrophoresis and also by hemagglutination. Three major extracellular protein components were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the effects of growth conditions on these components were determined. Results for batch-grown cultures showed that there was genotypic variation in the susceptibility of cells to lysis by a muramidase. The enhancement of lipoteichoic acid production by fructose and sorbitol in batch cultures was not identical in representative strains of S. mutans serotype c, nor was the effect of fructose found uniformly in representative strains of the different S. mutans serotypes.
Authors:
L Hardy; N A Jacques; H Forester; L K Campbell; K W Knox; A J Wicken
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  31     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1981 Jan 
Date Detail:
Created Date:  1981-06-23     Completed Date:  1981-06-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  78-87     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / metabolism*
Bacteriolysis
Carbohydrates / pharmacology
Culture Media
Fructose / pharmacology*
Glucose / pharmacology
Lipopolysaccharides*
Phosphatidic Acids / biosynthesis*
Sorbitol / pharmacology
Streptococcus mutans / growth & development,  metabolism*
Surface Properties
Teichoic Acids / biosynthesis*
Grant Support
ID/Acronym/Agency:
R01 DEO 4174/DE/NIDCR NIH HHS; R01 DEO 4175/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Carbohydrates; 0/Culture Media; 0/Lipopolysaccharides; 0/Phosphatidic Acids; 0/Teichoic Acids; 30237-26-4/Fructose; 50-70-4/Sorbitol; 50-99-7/Glucose; 56411-57-5/lipoteichoic acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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