Document Detail


Effect of freezing and storing on the biological properties of macrophages.
MedLine Citation:
PMID:  1171143     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Storage of intact macrophages would be a useful procedure for laboratories studying the biological functions of these cells. Oil-induced guinea pig peritoneal cells were harvested and frozen at an ultra-low temperature using a cryoprotective media. Studies of the biological properties of the frozen cells upon thawing indicated that the cells retained viability for a fairly long period of time, were metabolically active and comparable to normal cells in morphology. Indications of activation were obtained in enhanced phagocytic activity and significant decrease in migration of the stored cells.
Authors:
R Ganguly; D Miller; K P Czaruk; V Senterfitt; R H Waldman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunological methods     Volume:  8     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1975  
Date Detail:
Created Date:  1975-11-08     Completed Date:  1975-11-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  101-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Ascitic Fluid / cytology
Bacteriolysis
Butyrates / metabolism
Carbon Radioisotopes
Cell Migration Inhibition
Cell Survival
Cell-Free System
Freezing*
Guinea Pigs / immunology
Macrophages* / immunology,  metabolism
Phagocytosis
Preservation, Biological / methods*
Staphylococcus / immunology
Chemical
Reg. No./Substance:
0/Butyrates; 0/Carbon Radioisotopes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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