Document Detail

Effect of freeze-thaw cycles on serum measurements of AFP, CEA, CA125 and CA19-9.
MedLine Citation:
PMID:  17852813     Owner:  NLM     Status:  MEDLINE    
AFP, CEA, CA125 and CA19-9 are commonly used serum tumour markers (TMs) in clinical practice, although their quantification by immunoassay may be influenced by pre-analytical sample handling. Though the effect of repetitive freeze-thaw cycles is generally recognized, it is not clear in detail. The present study measured (CLIA) these TMs in serum samples freshly separated and after each of five freeze-thaw cycles, in which the samples were frozen at -40 degrees C for 10 months at cycle 4 and 2 h at other cycles. Statistical analysis with the General Linear Model for Repeated Measures revealed significant decreases in the measurements of the four TMs, with the least decrease of 6.8 % for CA125 and the most decrease of 18.2 % for CA19-9 after the last cycle, and an overwhelming single cycle decrease of mean 7.7 % at cycle 4 for AFP, CEA and CA125, of 7.5 % and 9.3 % at cycles 4 and 5 for CA19-9. So it seems that measurements of AFP, CEA and CA125 are more readily affected by long-term frozen storage compared with frequent freezing-thawing, while CA19-9 is relatively unstable under both conditions.
Y-C Gao; Z-B Yuan; Y-D Yang; H-K Lu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Scandinavian journal of clinical and laboratory investigation     Volume:  67     ISSN:  0036-5513     ISO Abbreviation:  Scand. J. Clin. Lab. Invest.     Publication Date:  2007  
Date Detail:
Created Date:  2007-12-05     Completed Date:  2008-01-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404375     Medline TA:  Scand J Clin Lab Invest     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  741-7     Citation Subset:  IM    
The Immunoassay Laboratory, Department of Nuclear Medicine, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China.
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MeSH Terms
CA-125 Antigen / blood*
CA-19-9 Antigen / blood*
Carcinoembryonic Antigen / blood*
Middle Aged
Neoplasms / blood
Reproducibility of Results
Specimen Handling / methods*
Tumor Markers, Biological / blood
alpha-Fetoproteins / analysis*
Reg. No./Substance:
0/CA-125 Antigen; 0/CA-19-9 Antigen; 0/Carcinoembryonic Antigen; 0/Tumor Markers, Biological; 0/alpha-Fetoproteins

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