| Effect of freeze-thaw cycles on serum measurements of AFP, CEA, CA125 and CA19-9. | |
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MedLine Citation:
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PMID: 17852813 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AFP, CEA, CA125 and CA19-9 are commonly used serum tumour markers (TMs) in clinical practice, although their quantification by immunoassay may be influenced by pre-analytical sample handling. Though the effect of repetitive freeze-thaw cycles is generally recognized, it is not clear in detail. The present study measured (CLIA) these TMs in serum samples freshly separated and after each of five freeze-thaw cycles, in which the samples were frozen at -40 degrees C for 10 months at cycle 4 and 2 h at other cycles. Statistical analysis with the General Linear Model for Repeated Measures revealed significant decreases in the measurements of the four TMs, with the least decrease of 6.8 % for CA125 and the most decrease of 18.2 % for CA19-9 after the last cycle, and an overwhelming single cycle decrease of mean 7.7 % at cycle 4 for AFP, CEA and CA125, of 7.5 % and 9.3 % at cycles 4 and 5 for CA19-9. So it seems that measurements of AFP, CEA and CA125 are more readily affected by long-term frozen storage compared with frequent freezing-thawing, while CA19-9 is relatively unstable under both conditions. |
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Authors:
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Y-C Gao; Z-B Yuan; Y-D Yang; H-K Lu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Scandinavian journal of clinical and laboratory investigation Volume: 67 ISSN: 0036-5513 ISO Abbreviation: Scand. J. Clin. Lab. Invest. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-12-05 Completed Date: 2008-01-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0404375 Medline TA: Scand J Clin Lab Invest Country: Norway |
Other Details:
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Languages: eng Pagination: 741-7 Citation Subset: IM |
Affiliation:
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The Immunoassay Laboratory, Department of Nuclear Medicine, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China. gaookok@163.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged CA-125 Antigen / blood* CA-19-9 Antigen / blood* Carcinoembryonic Antigen / blood* Female Freezing Humans Male Middle Aged Neoplasms / blood Reproducibility of Results Specimen Handling / methods* Tumor Markers, Biological / blood alpha-Fetoproteins / analysis* |
| Chemical | |
Reg. No./Substance:
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0/CA-125 Antigen; 0/CA-19-9 Antigen; 0/Carcinoembryonic Antigen; 0/Tumor Markers, Biological; 0/alpha-Fetoproteins |
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