Document Detail


Effect of freeze drying and protectants on viability of the biocontrol yeast Candida sake.
MedLine Citation:
PMID:  11393686     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of freezing method, freeze drying process, and the use of protective agents on the viability of the biocontrol yeast Candida sake were studied. Freezing at -20 degrees C was the best method to preserve the viability of C. sake cells after freeze drying using 10% skim milk as a protectant (28.9% survival). Liquid nitrogen freezing caused the highest level of damage to the cells with viability < 10%. Different concentrations of exogenous substances including sugars, polyols, polymers and nitrogen compounds were tested either alone or in combination with skim milk. There was little or no effect when additives were used at 1% concentration. Galactose, raffinose and sodium glutamate at 10% were the best protective agents tested alone but the viability of freeze-dried C. sake cells was always < 20%. Survival of yeast cells was increased from 0.2% to 30-40% by using appropriate protective media containing combinations of skim milk and other protectants such as 5% or 10% lactose or glucose, and 10% fructose or sucrose.
Authors:
M Abadias; A Benabarre; N Teixidó; J Usall; I Viñas
Related Documents :
3595736 - A method for freezing synchronous mitotic and g1 cells.
13646626 - Isotope dilution method for assay of inagglutinable erythrocytes.
6163216 - Induction of hemoglobin accumulation in human k562 cells by hemin is reversible.
6623846 - Erythrocyte volume distribution analysis and hematologic changes in two horses with imm...
20082656 - Discrepancies between metabolic activity and dna content as tool to assess cell prolife...
8886576 - Erythropoietin stimulates proliferation of rat-cultured gastric mucosal cells.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of food microbiology     Volume:  65     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-06-06     Completed Date:  2001-06-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  173-82     Citation Subset:  IM    
Affiliation:
Postharvest Unit, CeRTA Centre UdL-IRTA, Lleida, Catalonia, Spain. isabel.abadias@irta.es
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Candida / drug effects*,  growth & development*
Cryoprotective Agents / pharmacology*
Disaccharides / pharmacology
Food Handling*
Food Microbiology
Freeze Drying*
Milk
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 0/Disaccharides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Genetic identification of Saccharomyces bayanus var. uvarum, a cider-fermenting yeast.
Next Document:  Characterization of Penicillium roqueforti strains used as cheese starter cultures by RAPD typing.