| Effect of free radicals on pulmonary vascular response to acetylcholine. | |
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MedLine Citation:
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PMID: 1757317 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe a model of pulmonary endothelial injury caused by electrolysis-generated free radicals. Rabbit lungs were perfused in situ with Krebs solution at 37 degrees C containing 30 microM indomethacin. Electrolysis of this solution for 2 min, with a constant DC current of 20 mA, caused pulmonary vasoconstriction during the passage of current and converted subsequent acetylcholine-induced vasodilation to vasoconstriction. Electrolysis also inhibited endothelial-dependent vasodilation due to the calcium ionophore A23187 but not that due to sodium nitroprusside, suggesting that smooth muscle function was unaltered, while that of the endothelium of the lung is specifically modified by the stimulus. These effects were prevented by a mixture of superoxide dismutase and catalase or by sodium salicylate, which removes hydroxyl radicals from solution after electrolysis. Electrolysis-induced endothelial damage was less functionally obvious when electrolysis was applied during recirculation of Krebs solution, perhaps because recirculating perfusion may trigger release of either free radical scavengers or other protective substances. This technique offers a simple reproducible model to study free radical-related damage of endothelium in the intact lung. |
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Authors:
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X Chen; C N Gillis |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 71 ISSN: 8750-7587 ISO Abbreviation: J. Appl. Physiol. Publication Date: 1991 Sep |
Date Detail:
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Created Date: 1992-01-31 Completed Date: 1992-01-31 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 821-5 Citation Subset: IM |
Affiliation:
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Department of Anesthesiology, Yale University School of Medicine, New Haven, Connecticut 06510. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid Acetylcholine / pharmacology* Animals Atropine / pharmacology Calcimycin / pharmacology Catalase / pharmacology Deferoxamine / pharmacology Endothelium, Vascular / drug effects, physiology Free Radicals Male Muscle, Smooth, Vascular / drug effects, physiology Nitroprusside / pharmacology Perfusion Prostaglandin Endoperoxides, Synthetic / pharmacology Pulmonary Circulation / drug effects* Rabbits Sodium Salicylate / pharmacology Superoxide Dismutase / pharmacology Vasoconstriction / drug effects Vasodilation / drug effects |
| Grant Support | |
ID/Acronym/Agency:
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HL-13315/HL/NHLBI NIH HHS; HL-40863/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Free Radicals; 0/Prostaglandin Endoperoxides, Synthetic; 15078-28-1/Nitroprusside; 51-55-8/Atropine; 51-84-3/Acetylcholine; 52665-69-7/Calcimycin; 54-21-7/Sodium Salicylate; 70-51-9/Deferoxamine; 76898-47-0/15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; EC 1.11.1.6/Catalase; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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