Document Detail


Effect of free fatty acids and lysolipids on cellular uptake of doxorubicin in human breast cancer cell lines.
MedLine Citation:
PMID:  20559126     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several fatty acids and lysolipids have been shown earlier to increase the permeability of membranes of artificial liposomes, thereby increasing the release of drugs such as doxorubicin (Dox) contained within them. Free fatty acids can also inhibit cancer cell growth in vitro, and it has been suggested that this inhibition results from increased membrane permeability. Clearly, therefore, increased membrane permeability could be used in the design of liposomes for targeted drug delivery. For example, as free fatty acids and lysolipids are released upon phospholipase degradation of the liposome, the liposome could deliver membrane permeability enhancers in addition to the drug to increase the targeted anticancer effect. In this study, we examined the effect on Dox uptake in the breast cancer cell lines MDA-MB-231, MCF7, and MCF7-MDR when incubated with a large panel of different free fatty acids and lysolipids. Dox uptake was quantified by flow cytometry and fluorescence microscopy. We observed no increased Dox uptake in any of the breast cancer cell lines, suggesting that growth inhibitory effects observed earlier subsequent to the addition of free fatty acids to cancer cells are not caused by increased cell membrane permeability.
Authors:
Nicolaj Rasmussen; Jonas H Andersen; Henrik Jespersen; Ole G Mouritsen; Henrik J Ditzel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anti-cancer drugs     Volume:  21     ISSN:  1473-5741     ISO Abbreviation:  Anticancer Drugs     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-08     Completed Date:  2010-08-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9100823     Medline TA:  Anticancer Drugs     Country:  England    
Other Details:
Languages:  eng     Pagination:  674-7     Citation Subset:  IM    
Affiliation:
Department of Cancer and Inflammation Research, Institute of Molecular Medicine, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Antibiotics, Antineoplastic / metabolism*
Breast Neoplasms / metabolism*
Cell Line, Tumor
Cell Membrane Permeability / drug effects
Doxorubicin / metabolism*
Drug Screening Assays, Antitumor
Fatty Acids, Nonesterified / pharmacology*
Female
Humans
Lipids / pharmacology*
Chemical
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/Fatty Acids, Nonesterified; 0/Lipids; 23214-92-8/Doxorubicin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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