Document Detail

Effect of free amino acids and vitamins on cleavage and developmental rate of bovine zygotes in vitro.
MedLine Citation:
PMID:  8157527     Owner:  NLM     Status:  MEDLINE    
Due to the complicated media used for culturing bovine embryos, most of the nutrient requirements are unknown. Recently, we developed a simple, serum-free medium (CR1) that allows bovine embryos to develop in vitro. Therefore, our objective was to determine whether development of bovine embryos would be improved by the addition of free amino acids and vitamins to CR1. Oocytes were recovered from slaughterhouse ovaries and matured 22 +/- 2 h, following which the oocytes were randomly allotted to treatment. The experiment was a randomized block design with a 2 x 5 factorial treatment structure. The oocytes were fertilized with or without cumulus cells intact. The five fertilization media were 1) Control (CR1 +/- 10 micrograms/mL of phenol red); 2) control + basal medium Eagle (BME) essential amino acids (EAA) + minimum essential medium (MEM) nonessential amino acids (NEA) + MEM vitamins (VIT); 3) control + EAA + NEA; 4) control + EAA + VIT; and 5) control + NEA + VIT. Cleavage rate was greater (P < .001) when cumulus cells remained on the oocytes during fertilization (51.7 vs 73.2% without and with cumulus cells, respectively). The frequency of blastocysts was increased (P < .001) when EAA or NEA were added to CR1; however, adding VIT had no effect or tended (P = .12) to decrease the frequency of embryos attaining the blastocyst stage. This experiment demonstrates that development of bovine embryos in vitro can be improved by the addition of free amino acids to a simple medium. Contrary to work in rodents, the mixture of vitamins in MEM was not beneficial for bovine embryos.
C F Rosenkrans; N L First
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of animal science     Volume:  72     ISSN:  0021-8812     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-05-17     Completed Date:  1994-05-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  434-7     Citation Subset:  IM    
Department of Animal Science, University of Arkansas, Fayetteville 72701.
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MeSH Terms
Amino Acids / pharmacology*
Cattle / embryology*
Cleavage Stage, Ovum / drug effects
Culture Media
Fertilization in Vitro
Random Allocation
Vitamins / pharmacology*
Zygote / drug effects*,  growth & development
Reg. No./Substance:
0/Amino Acids; 0/Culture Media; 0/Vitamins

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