| Effect of fouling on the capacity and breakthrough characteristics of a packed bed ion exchange chromatography column. | |
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MedLine Citation:
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PMID: 16450113 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study examined the impact of fouling with yeast homogenate on capacity and breakthrough performance of an ion exchange packed bed column. Column performance was assessed by analysis of breakthrough curves obtained with BSA as a test protein. The overall impact of fouling on breakthrough performance depended heavily on the level of clarification of the feed stream. Challenging the column with particulate-free homogenate caused no change in column performance. Loading successive small volumes of poorly clarified homogenate, interspersed with frequent column salt washes, did not alter significantly the column capacity. By contrast, when the column was challenged with an equivalent cumulative volume of poorly clarified homogenate, dynamic binding capacity decreased significantly and changes in breakthrough curves suggested increased intraparticle and external mass transfer limitations. These changes were ascribed to deposition of solid particulates in void spaces in the bed and colloidal contaminants in the bead pores. |
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Authors:
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Sun Chau Siu; Helen Baldascini; David C Hearle; Mike Hoare; Nigel J Titchener-Hooker |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-02-01 |
Journal Detail:
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Title: Bioprocess and biosystems engineering Volume: 28 ISSN: 1615-7591 ISO Abbreviation: Bioprocess Biosyst Eng Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-06-15 Completed Date: 2006-07-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101088505 Medline TA: Bioprocess Biosyst Eng Country: Germany |
Other Details:
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Languages: eng Pagination: 405-14 Citation Subset: IM |
Affiliation:
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The Advanced Centre for Biochemical Engineering, University College London, Torrington Place, WC1E 7JE, London, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Ion Exchange
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instrumentation* Equipment Contamination / prevention & control* Equipment Failure* Equipment Failure Analysis* Saccharomyces cerevisiae / isolation & purification* Ultrafiltration / instrumentation* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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