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Effect of formoterol on eosinophil trans-basement membrane migration induced by interleukin-8-stimulated neutrophils.
MedLine Citation:
PMID:  23711848     Owner:  NLM     Status:  In-Data-Review    
Background: Neutrophils are often increased in the airways of either chronic severe asthma or acute exacerbations. Neutrophils that have migrated in response to interleukin-8 (IL-8) may lead eosinophils to accumulate in the airways of patients with asthma and possibly aggravate the disease. In this study, we investigated whether formoterol modified the trans-basement membrane migration (TBM) of eosinophils stimulated with neutrophils and IL-8. Methods: Neutrophils and eosinophils were isolated from peripheral blood obtained from healthy donors. Eosinophil TBM was examined using a modified Boyden's chamber technique. Neutrophils were preincubated with or without formoterol (0.1 μM) at 37°C for 30 min. Eosinophils were added to the upper compartment of a chamber with a Matrigel-coated transwell insert. Medium containing preincubated neutrophils and IL-8 was added to the lower compartment of the chamber. After a 90-minute incubation, the eosinophils that had migrated into the lower chamber were calculated using eosinophil peroxidase assays. Results: A combination of neutrophils and IL-8 significantly induced the eosinophil TBM; formoterol alone had no effect. However, formoterol modestly but significantly attenuated the TBM of eosinophils stimulated with neutrophils and IL-8. Conclusion: These results suggest that formoterol may act as a therapeutic agent on enhanced eosinophilic inflammation in acute exacerbation or persistent, severe asthma. The effect of formoterol likely involves the inhibition of neutrophil activation.
Akiko Kawashima; Tomoko Suzuki; Fuyumi Nishihara; Takehito Kobayashi; Yotaro Takaku; Kazuyuki Nakagome; Tomoyuki Soma; Koichi Hagiwara; Minoru Kanazawa; Makoto Nagata
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Publication Detail:
Type:  Journal Article     Date:  2013-05-29
Journal Detail:
Title:  International archives of allergy and immunology     Volume:  161 Suppl 2     ISSN:  1423-0097     ISO Abbreviation:  Int. Arch. Allergy Immunol.     Publication Date:  2013  
Date Detail:
Created Date:  2013-05-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9211652     Medline TA:  Int Arch Allergy Immunol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  10-5     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 S. Karger AG, Basel.
Department of Respiratory Medicine, Saitama Medical University, Saitama, Japan.
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