| Effect of food restriction on the phosphocreatine energy shuttle components in rat heart. | |
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MedLine Citation:
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PMID: 1433312 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Malnutrition has been associated with changes in cardiac metabolism and performance. We have previously reported a diabetic-type cardiomyopathy associated with chronic food restriction and weight loss. Because the creatine-phosphocreatine-creatine kinase system is important in the contractile process, we studied the components of this system in rats fed a food-restricted diet (33% of control animal intake). After 4 weeks of food restriction, total creatine kinase (CK) activities were reduced by 28% in ventricles and by 38% in atria. The CK isoenzymes in the heart were not equally affected. The BB isoenzyme was decreased by 77% and 78%, the MB isoenzyme by 45% and 43%, the MM isoenzyme by 22% and 19% and CKmito by 16% and 15% in ventricles and atria, respectively. In contrast, brain CK activity which is predominantly the BB isoenzyme, was slightly higher in the food-restricted than in control rats. Further studies on ventricular tissue from food-restricted rats revealed a 27% decline in myofibrillar CR activity and a 58% decline in myofibrillar ATPase activity. Phosphocreatine and creatine concentrations were not changed by food restriction, however, ATP was decreased by 23% in ventricles from rats on the restricted diet. Cardiac mitochondrial oxidative phosphorylation was also impaired. State 3 respiration with alpha-ketoglutarate was reduced 20% in the food-restricted heart. These changes are compared to those which we previously observed in the diabetic rat heart and the significance of these findings is discussed. |
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Authors:
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A Kirsch; F Savabi |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of molecular and cellular cardiology Volume: 24 ISSN: 0022-2828 ISO Abbreviation: J. Mol. Cell. Cardiol. Publication Date: 1992 Aug |
Date Detail:
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Created Date: 1992-12-18 Completed Date: 1992-12-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0262322 Medline TA: J Mol Cell Cardiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 821-30 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Nutrition, University of Southern California, School of Medicine, Los Angeles 90033. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphatases
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metabolism Adenylate Kinase / metabolism Animals Creatine Kinase / metabolism Energy Metabolism Food Deprivation / physiology* Isoenzymes Male Mitochondria, Heart / metabolism Myocardium / metabolism* Nutrition Disorders / metabolism Oxidative Phosphorylation Phosphocreatine / metabolism* Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 67-07-2/Phosphocreatine; EC 2.7.3.2/Creatine Kinase; EC 2.7.4.3/Adenylate Kinase; EC 3.6.1.-/Adenosine Triphosphatases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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