Document Detail

Effect of food restriction on the phosphocreatine energy shuttle components in rat heart.
MedLine Citation:
PMID:  1433312     Owner:  NLM     Status:  MEDLINE    
Malnutrition has been associated with changes in cardiac metabolism and performance. We have previously reported a diabetic-type cardiomyopathy associated with chronic food restriction and weight loss. Because the creatine-phosphocreatine-creatine kinase system is important in the contractile process, we studied the components of this system in rats fed a food-restricted diet (33% of control animal intake). After 4 weeks of food restriction, total creatine kinase (CK) activities were reduced by 28% in ventricles and by 38% in atria. The CK isoenzymes in the heart were not equally affected. The BB isoenzyme was decreased by 77% and 78%, the MB isoenzyme by 45% and 43%, the MM isoenzyme by 22% and 19% and CKmito by 16% and 15% in ventricles and atria, respectively. In contrast, brain CK activity which is predominantly the BB isoenzyme, was slightly higher in the food-restricted than in control rats. Further studies on ventricular tissue from food-restricted rats revealed a 27% decline in myofibrillar CR activity and a 58% decline in myofibrillar ATPase activity. Phosphocreatine and creatine concentrations were not changed by food restriction, however, ATP was decreased by 23% in ventricles from rats on the restricted diet. Cardiac mitochondrial oxidative phosphorylation was also impaired. State 3 respiration with alpha-ketoglutarate was reduced 20% in the food-restricted heart. These changes are compared to those which we previously observed in the diabetic rat heart and the significance of these findings is discussed.
A Kirsch; F Savabi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of molecular and cellular cardiology     Volume:  24     ISSN:  0022-2828     ISO Abbreviation:  J. Mol. Cell. Cardiol.     Publication Date:  1992 Aug 
Date Detail:
Created Date:  1992-12-18     Completed Date:  1992-12-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0262322     Medline TA:  J Mol Cell Cardiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  821-30     Citation Subset:  IM    
Department of Pharmacology and Nutrition, University of Southern California, School of Medicine, Los Angeles 90033.
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MeSH Terms
Adenosine Triphosphatases / metabolism
Adenylate Kinase / metabolism
Creatine Kinase / metabolism
Energy Metabolism
Food Deprivation / physiology*
Mitochondria, Heart / metabolism
Myocardium / metabolism*
Nutrition Disorders / metabolism
Oxidative Phosphorylation
Phosphocreatine / metabolism*
Rats, Sprague-Dawley
Reg. No./Substance:
0/Isoenzymes; 67-07-2/Phosphocreatine; EC Kinase; EC Kinase; EC 3.6.1.-/Adenosine Triphosphatases

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