|Effect of food residues on norovirus survival on stainless steel surfaces.|
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|PMID: 21887215 Owner: NLM Status: MEDLINE|
|BACKGROUND: In households and food processing plants, minute food residues left behind from improper cleaning may influence the survivability of human norovirus on surfaces. In this study, the survivability of norovirus on desiccated food residue-attached stainless steel coupons was investigated.
METHODOLOGY/PRINCIPAL FINDINGS: Using murine norovirus-1 (MNV-1) as a surrogate of human norovirus, the survivability of norovirus was investigated on lettuce, cabbage, or ground pork-attached stainless steel coupons. A 6.2 log MPN/ml of MNV-1 infectivity was completely lost at day 30 in residue-free coupons, whereas only a 1.4 log MPN/ml reduction was observed in coupons with residues. Moreover, the disinfective effect of sodium hypochlorite was reduced when residues were present on the coupons.
CONCLUSIONS/SIGNIFICANCE: This study revealed that the food residues increased the survivability and the resistance to chemicals of norovirus, indicating the need of thorough cleaning in food processing plants and household settings.
|Hajime Takahashi; Ayumi Ohuchi; Satoko Miya; Yukino Izawa; Bon Kimura|
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|Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-08-22|
|Title: PloS one Volume: 6 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2011|
|Created Date: 2011-09-02 Completed Date: 2012-01-11 Revised Date: 2013-06-27|
Medline Journal Info:
|Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States|
|Languages: eng Pagination: e21951 Citation Subset: IM|
|Department of Food Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology, Tokyo, Japan.|
|APA/MLA Format Download EndNote Download BibTex|
Food Contamination / analysis*
Microbial Viability / drug effects*
Norovirus / drug effects*
Sodium Hypochlorite / pharmacology
Stainless Steel / pharmacology*
Surface Properties / drug effects
|0/Waste Products; 12597-68-1/Stainless Steel; 7681-52-9/Sodium Hypochlorite|
Journal ID (nlm-ta): PLoS One
Journal ID (publisher-id): plos
Journal ID (pmc): plosone
Publisher: Public Library of Science, San Francisco, USA
Takahashi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Received Day: 5 Month: 1 Year: 2011
Accepted Day: 15 Month: 6 Year: 2011
collection publication date: Year: 2011
Electronic publication date: Day: 22 Month: 8 Year: 2011
Volume: 6 Issue: 8
E-location ID: e21951
PubMed Id: 21887215
Publisher Id: PONE-D-11-00765
|Effect of Food Residues on Norovirus Survival on Stainless Steel Surfaces Alternate Title:Norovirus Survival|
|Department of Food Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology, Tokyo, Japan
|University of Cambridge, United Kingdom
|Correspondence: * E-mail: email@example.com.
Contributed by footnote: Conceived and designed the experiments: HT AO SM BK. Performed the experiments: HT AO SM YI. Analyzed the data: HT AO SM BK YI. Contributed reagents/materials/analysis tools: HT BK. Wrote the paper: HT SM BK.
Norovirus is a member of the Caliciviridae family, and causes gastroenteritis in humans. It is estimated that more than 50% of foodborne outbreaks in the United States may be attributable to norovirus . Likewise, in Japan, this virus accounted for a large portion (about 29%) of the foodborne diseases in 2009 . Besides ingestion of contaminated foods, such as raw oysters, person-to-person transmission can occur, or transmission may occur via contaminated surfaces and aerosols . The infective dose of this virus is very low; even 10 viral particles are sometimes enough to infect an individual .
Foodborne outbreaks of norovirus are often associated with infected food handlers in food processing plants –. Therefore, in this study, we investigated the survivability of norovirus on stainless steel, which is a common surface material in food processing plants. Although the efficiency of norovirus attachment to stainless steel surfaces has been studied previously , no studies have assessed the survivability of this virus on desiccated surfaces. Notably, the effect of minute food residues on virus survivability, on food-contact surfaces, is of interest in terms of food hygiene in food processing plants and improperly cleaned households. We, therefore, examined the virus survivability by attaching lettuce, cabbage, and pork filtrates on stainless steel coupons. Leafy greens such as lettuce, cabbage, and spinach are increasingly associated with foodborne outbreaks. According to CDC data , 502 (4.8%) outbreaks, 18,242 (6.5%) illnesses, and 15 (4.0%) deaths among 10,421 foodborne outbreaks reported during 1973–2006 were caused by leafy greens, and norovirus was responsible for 196 (58.3%) of these outbreaks. In addition, pork was used for a proteinaceous food since food processing plants usually handle many kinds of foods and this kind of food should be investigated for norovirus survivability as well as leafy greens. Moreover, we investigated the effect of sodium hypochlorite on norovirus inactivation on stainless steel surfaces, since this is the preventive measure recommended by the Japanese Ministry of Health, Labour and Welfare.
Although it is imperative that human norovirus is studied in order to prevent further foodborne infections, this virus is uncultivable in the laboratory. Thus, feline calicivirus (FCV) has been widely used as a surrogate in inactivation studies –, including inactivation on food-contact surfaces . However, FCV belongs to a different genus, Vesivirus, and is known as a respiratory pathogen . Among genus Norovirus, porcine norovirus and bovine norovirus are currently unable to be cultured in vitro, and moreover, porcine and bovine are difficult to genetically manipulate as animal models . On the other hand, murine norovirus-1 (MNV-1), first isolated from the brain of immunodeficient mouse after intracerebral inoculation in 2003 , is now accepted as a surrogate for human norovirus –. MNV-1 is the only norovirus that replicates in cell culture , and it is associated with gastrointestinal disease in addition to respiratory infection , , having low-pH tolerance  just like human norovirus . MNV-1 as a model for human norovirus has provided new insight into norovirus lifecycle and interaction with their host. In this study, therefore, we used MNV-1 as a surrogate for human norovirus.
We investigated survival and disinfection of human norovirus on food contact surfaces using MNV-1 as an experimental surrogate. First of all, MNV-1 infectivity on stainless steel with or without food residues was investigated (Fig. 1). On the stainless steel coupons without any food residue, MNV-1 infectivity rapidly decreased by more than 2 log MPN/ml, followed by a slow decline and a complete loss at day 30 (the 30-day reduction was significant with P<0.05). On the other hand, MNV-1 infectivity on stainless steel coupons with food residues (lettuce, cabbage, and ground pork) decreased by approximately 1.4 log MPN/ml by day 9 and remained at the same level for the rest of the experimental period (the 30-day reduction was not statistically significant with P>0.05). Previously better survivability of norovirus in ham than in lettuce and strawberry was reported . The authors ascribed this result to the pH difference of the foods. In our study, we had little difference in norovirus survivability among foodstuffs, possibly because the pH of the food extracts did not differ much from each other (6.89, 6.90, and 6.20 for lettuce, cabbage, and pork, respectively). The difference in norovirus survivability depending on the presence of food residues indicated that the presence of food residues increases the resistance to drying, thus thorough cleanliness in food processing plants is important in preventing foodborne infections. Moreover, if the surfaces were not completely dry, the survivability would be higher , suggesting a need for inactivation efforts.
In Japan, sodium hypochlorite is a government-recommended chemical for norovirus inactivation. The effect of various concentrations of sodium hypochlorite on MNV-1 inactivation is shown in Fig. 2. When no sodium hypochlorite was added to stainless steel coupons with or without food residues as controls, there was no reduction of MNV-1 infectivity (data not shown). Sodium hypochlorite at 1000 ppm was sufficient to inactivate the virus in the absence of food residues. On the other hand, even 2000 ppm had little effect on MNV-1 infectivity on stainless steel coupons with food residues. This is additional evidence of the influence of food components on virus survival. In order to confirm that the virus particles were not washed off but the infectivity was inactivated by the addition of the sodium hypochlorite, real-time reverse transcription PCR was conducted according to the method previously described . The similar RNA level was observed on coupons with or without sodium hypochlorite (data not shown), supporting the inactivation effect of sodium hypochlorite. Girard et al.  demonstrated that among major household disinfectants and neutralizers, only sodium hypochlorite was effective against norovirus, and other studies also affirmed the validity of this agent , . Our results are also in agreement with these studies only in the absence of food residues. A higher concentration of sodium hypochlorite than is recommended by the government (200 ppm) or the use of other chemical agents should be considered for norovirus inactivation on food contact surfaces in case of the presence of food residues.
Norovirus is often found in fecal material and vomit of infected people, and they are the important sources of spreading viruses. As a consequence, the survivability of the virus in these materials has been previously reported . This study, on the other hand, focused on the survivability on stainless steel which is often used in food processing plants as kitchen utensils and countertop, because the contamination in plants has been often the cause of norovirus outbreak. To our knowledge, this is the first study investigating the effect of food residues on norovirus survivability, which simulates conditions found in food processing plants and households with insufficient cleaning. We found that food residues had a significant effect on norovirus survival, increasing both survivability and chemical resistance. This study reveals that an appropriate and thorough cleaning procedure is imperative to prevent norovirus contamination.
RAW 264.7 cells were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA), and maintained as described by Wobus et al. . Murine norovirus strain 1 (MNV-1), kindly provided by Dr. Herbert W. Virgin from Washington University, was inoculated into RAW 264.7 cells and incubated at 37°C in 5% CO2 for 72 h. After 3 freeze-thaw cycles, MNV-1 was centrifuged at 8,000× g for 20 min and stored at −80°C until use.
To 100 g of each sample (lettuce, cabbage, or ground pork), an equal amount of sterile phosphate buffered saline (PBS) was added, and the resulting sample was homogenized for 1 min using a Stomacher. The sample was centrifuged (3,500× g for 10 min), and after filtering the supernatant using Advantec filter paper (No. 2, Toyo Roshi, Tokyo, Japan), the sample was filter-sterilized using a membrane filter unit (0.2-µm pore size, Nalgene Labware, Rochester, NY). Stainless steel coupons (2.5×7.5 cm, #400 polish) were autoclaved, washed with distilled water, and sonicated for 1 h, followed by dry-heat sterilization at 180°C for 2 h. The resulting stainless steel coupons were soaked in a sterile food filtrate, quickly removed and then dried on a clean bench. For comparison, stainless steel residue-free coupons were prepared.
Twenty microliters of MNV-1 at 7.9 log MPN/ml (6.2 log MPN) was inoculated onto each of the above-mentioned stainless steel coupons, air-dried and stored up to 30 days inside centrifugation tubes at 25°C. Stainless steel coupons were collected every 3 days. The viral particles were eluted by adding 1 ml of 0.05 M glycine buffer and obtained by scraping the stainless steel coupons with cell scrapers. Viral infectivity was determined by the most probable number method (MPN) described below. MPN was done in triplicate.
Twenty microliters of MNV-1 at 7.9 log MPN/ml (6.2 log MPN) was inoculated onto each of the above-mentioned stainless steel coupons with or without food residues. After 30-min air-drying, 80 µl of various concentrations (200, 500, 1000, and 2000 ppm) of sodium hypochlorite was added. As negative controls, additional stainless steel coupons with or without food residues were prepared for no sodium hypochlorite addition. After a 5-min exposure, the sodium hypochlorite was neutralized by 0.01 N Na2S2O3 supplemented with 10% fetal bovine serum/Dulbecco's modified Eagle's medium (FBS-DMEM), and MNV-1 was recovered with cell scrapers. The infectivity was determined by MPN method as well. MPN was done in triplicate.
The viral infectivity was determined by MPN method with 95% confidence limits by the 5-tube method. MPN is a statistical tool, deducing the “most probable number” of microorganisms based on the proportion of viral-positive plates to the total number of plates after serial dilutions by factors of ten . This method provides accurate results  and is used widely in some fields of microbiological research, including those on norovirus , . Briefly, MNV-1 scraped from stainless steel coupons was serially diluted in 10% FBS-DMEM, then inoculated into RAW 264.7 at 2,250,000 cells/well. After a 5-day incubation at 37°C, plates were washed with 100% methanol and the cells were stained with 5% crystal violet. Unstained cells were considered to be dead, which means MNV-1 was present. From the number of MNV-1-positive wells among five wells at the appropriate dilution, the MPN was determined based on a conversion table .
For comparisons on virus survivability among treatments, statistical analysis was performed using the one-way analysis of variance with Tukey's multiple comparison test (SPSS version 17.0, SPSS Japan Inc., Tokyo, Japan).
Competing Interests: The authors have declared that no competing interests exist.
Funding: This study was supported by Tokyo University of Marine Science and Technology. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.
|1.||Centers for Disease Control and PreventionYear: 2010Norovirus: Technical Fact Sheet. Available: http://www.cdc.gov/ncidod/dvrd/revb/gastro/norovirus-factsheet.htm. Accessed 2010 Dec 10.|
|2.||Ministry of Health, Labour and WelfareYear: 2009Food Poisoning Surveillance Report. (In Japanese) Available: http://www.mhlw.go.jp/topics/syokuchu/04.html#4-2. Accessed 2011 June 3.|
|3.||Moe C,Sobsey MD,Stewart P,Crawford-Brown D. Year: 1999Estimating the risk of human calicivirus infection from drinking water. Presented at the First International Workshop on Human Calicivirus, Atlanta, Georgia.|
|4.||Friedman DS,Heisey-Grove D,Argyros F,Berl E,Nsubuga J,et al. Year: 2005An outbreak of norovirus gastroenteritis associated with wedding cakes.Epidemiol Infect1331057106316274502|
|5.||Kuritsky JN,Osterholm MT,Greenberg HB,Korlath JA,Godes JR,et al. Year: 1984Norwalk gastroenteritis: a community outbreak associated with bakery product consumption.Ann Intern Med1005195216322631|
|6.||Malek M,Barzilay E,Kramer A,Camp B,Jaykus LA,et al. Year: 2009Outbreak of norovirus infection among river rafters associated with packaged delicatessen meat, Grand Canyon, (2005).Clin Infect Dis48313719025489|
|7.||Parashar UD,Dow L,Fankhauser RL,Humphrey CD,Miller J,et al. Year: 1998An outbreak of viral gastroenteritis associated with consumption of sandwiches: implications for the control of transmission by food handlers.Epidemiol Infect12161562110030711|
|8.||Patterson W,Haswell P,Fryers PT,Green J. Year: 1997Outbreak of small round structured virus gastroenteritis arose after kitchen assistant vomited.Commun Dis Rep7R101R103|
|9.||Widdowson MA,Sulka A,Bulens SN,Beard RS,Chaves SS,et al. Year: 2005Norovirus and foodborne disease, United States, 1991–2000.Emerg Infect Dis119510215705329|
|10.||Girard M,Ngazoa S,Mattison K,Jean J. Year: 2010Attachment of noroviruses to stainless steel and their inactivation, using household disinfectants.J Food Prot7340040420132692|
|11.||Herman K,Ayers T,Lynch M. Year: 2008Foodborne disease outbreaks associated with leafy greens, 1973–2006. Presented at the International Conference on Emerging Infectious Diseases 2008, Atlanta, Georgia.|
|12.||Doultree JC,Druce JD,Birch CJ,Bowden DS,Marshall JA. Year: 1999Inactivation of feline calicivirus, a Norwalk virus surrogate.J Hosp Infect4151579949965|
|13.||Duizer E,Bijkerk P,Rockx B,de Groot A,Twisk F,et al. Year: 2004Inactivation of caliciviruses.Appl Environ Microbiol704538454315294783|
|14.||Urakami H,Ikarashi K,Okamoto K,Abe Y,Ikarashi T,et al. Year: 2007Chlorine sensitivity of feline calicivirus, a norovirus surrogate.Appl Environ Microbiol735679568217616623|
|15.||Whitehead K,McCue KA. Year: 2010Virucidal efficacy of disinfectant actives against feline calicivirus, a surrogate for norovirus, in a short contact time.Am J Infect Cont382630|
|16.||Gulati BR,Allwood PB,Hedberg CW,Goyal SM. Year: 2001Efficacy of commonly used disinfectants for the inactivation of calicivirus on strawberry, lettuce, and a food-contact surface.J Food Prot641430143411563523|
|17.||Hoover EA,Kahn DE. Year: 1975Experimentally induced feline calicivirus infection: clinical signs and lesions.J Am Vet Med Assoc1664634681112749|
|18.||Wobus CE,Thackray LB,Virgin HW. Year: 2006Murine norovirus: a model system to study norovirus biology and pathogenesis.J Virol805104511216698991|
|19.||Karst SM,Wobus CE,Lay M,Davidson J,Virgin HW. Year: 2003STAT1-dependent innate immunity to a Norwalk-like virus.Science2991575157812624267|
|20.||Bae J,Schwab KJ. Year: 2008Evaluation of murine norovirus, feline calicivirus, poliovirus, and MS2 as surrogates for human norovirus in a model of viral persistence in surface water and groundwater.Appl Environ Microbiol7447748418065626|
|21.||Cannon JL,Papafragkou E,Park GW,Osborne J,Jaykus LA,et al. Year: 2006Surrogates for the study of norovirus stability and inactivation in the environment: a comparison of murine norovirus and feline calicivirus.J Food Prot692761276517133824|
|22.||Wobus CE,Karst SM,Thackray LB,Chang KO,Sosnovtsev SV,et al. Year: 2004Replication of norovirus in cell culture reveals a tropism for dendritic cells and macrophages.PLoS Biol220762084|
|23.||Graham DY,Jiang X,Tanaka T,Opekun AR,Madore HP,et al. Year: 1994Norwalk virus infection of volunteers: new insights based on improved assays.J Infect Dis17034438014518|
|24.||Mattison K,Karthikeyan K,Abebe M,Malik N,Sattar SA,et al. Year: 2007Survival of calicivirus in foods and on surfaces: experiments with feline calicivirus as a surrogate for norovirus.J Food Prot7050050317340890|
|25.||Stals A,Baert L,Botteldoorn N,Werbrouck H,Herman L,et al. Year: 2009Multiplex real-time RT-PCR for simultaneous detection of GI/GII noroviruses and murine norovirus 1.J Virol Metthods161247253|
|26.||Belliot G,Lavaux A,Souihel D,Agnello D,Pothier P. Year: 2008Use of murine norovirus as a surrogate to evaluate resistance of human norovirus to disinfectants.Appl Environ Microbiol743315331818378650|
|27.||Park GW,Boston DM,Kase JA,Sampson MN,Sobsey MD. Year: 2007Evaluation of liquid- and fog-based application of Sterilox hypochlorous acid solution for surface inactivation of human norovirus.Appl Environ Microbiol734463446817483283|
|28.||Entis P. Year: 2002Most probable number (MPN).Entis P,Entis MFood microbiology: the laboratoryWashington DCFood Processor Institute1821|
|29.||Jimenez L,Chiang M. Year: 2006Virucidal activity of a quaternary ammonium compound disinfectant against feline calicivirus: A surrogate for norovirus.Am J Infect Control3426927316765204|
|30.||Katayama H,Haramoto E,Oguma K,Yamashita H,Tajima A,et al. Year: 2008One-year monthly quantitative survey of noroviruses, enteroviruses, and adenoviruses in wastewater collected from six plants in Japan.Water Res421441144817996920|
|31.||Garthright WE. Year: 1995Appendix 2. Most probable number from serial dilutions, p.2.08.Jackson GJFDA bacteriological analytical manualAOAC International, Washington, D.C.|
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