| Effect of folic acid on thymidylate synthase and thymidine kinase in regenerating rat liver after partial hepatectomy. | |
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MedLine Citation:
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PMID: 9528665 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of folic acid on liver regeneration after partial hepatectomy were investigated. The injection of folic acid inhibited the increases in the activities of thymidylate synthase and thymidine kinase in regenerating rat liver at 24 h after partial hepatectomy, with a concomitant reduction in DNA content. Northern blot analysis showed that this inhibition was due to the delay of the elevation of the mRNA levels of thymidylate synthase and thymidine kinase after partial hepatectomy. At 48 and 72 h, after partial hepatectomy, the thymidylate synthase activities in the folic acid injected rats increased to about 1.9- and 1.7-fold the corresponding control level, respectively, while thymidine kinase activities were similar to the control. Immunoblotting assay indicated that the increases in the thymidylate synthase activity at 48 and 72 h after partial hepatectomy were caused by a three fold increase in its protein level. Folic acid suppressed chymotryptic hydrolysis of thymidylate synthase. These suggest that folic acid increases the protein level of thymidylate synthase, at least in part, through protection against proteolysis. |
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Authors:
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M Komatsu; I Tsukamoto |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1379 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1998 Feb |
Date Detail:
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Created Date: 1998-04-21 Completed Date: 1998-04-21 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 289-96 Citation Subset: IM |
Affiliation:
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Department of Food Science and Nutrition, Nara Women's University, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chymotrypsin / metabolism DNA / metabolism Folic Acid / pharmacology* Hepatectomy Liver Regeneration / drug effects*, genetics, physiology Male Organ Size / drug effects Proteins / metabolism RNA / metabolism RNA, Messenger / genetics, metabolism Rats Rats, Wistar Thymidine Kinase / genetics, metabolism* Thymidylate Synthase / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Proteins; 0/RNA, Messenger; 59-30-3/Folic Acid; 63231-63-0/RNA; 9007-49-2/DNA; EC 2.1.1.45/Thymidylate Synthase; EC 2.7.1.21/Thymidine Kinase; EC 3.4.21.1/Chymotrypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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