Document Detail

Effect of flow on hydraulic conductivity and reflection coefficient of rabbit mesentery.
MedLine Citation:
PMID:  9866117     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To measure the hydraulic conductivity and reflection coefficient for albumin, as defined by the Starling equation, in rabbit mesentery. METHODS: A section of rabbit mesentery was fixed between two chambers filled with lactated Ringer solution. Flow (Q) of Ringer solution was measured across the mesentery at driving pressures (delta P) between 1 and 6 cm H2O. Hydraulic conductivity was proportional to the slope of the Q-delta P curve. At constant delta P (approximately -2, -4, or -6 cm H2O), flow was measured at three different albumin concentration differences (0.5, 1, and 1.5 g/dl). Unstirred layer effects were minimized by magnetic stirrers. Reflection coefficient was the negative of the slope of the Q-delta pi curve divided by the slope of the Q-delta P curve, where delta pi was the albumin osmotic pressure difference. Hydraulic conductivity was measured before and after testicular hyaluronidase was added to the Ringer solution. RESULTS: There was no significant difference in conductivity per unit area (Lp) for the three different driving pressures. Hyaluronidase increased hydraulic conductivity significantly (p < 0.01) by 72 +/- 56%, indicating that hyaluronan and other glycosaminoglycans contributed to tissue fluid resistance. Reflection coefficient (sigma) increased from 0.02 to 0.14 as flow increased eightfold. CONCLUSIONS: The results suggest that the mesentery tissue provides little restriction to the passage of albumin. The increase in conductivity in the presence of hyaluronidase indicates that tissue hyaluronan and other glycosaminoglycans provide fluid resistance to bulk flow.
S Parameswaran; L V Brown; S J Lai-Fook
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Microcirculation (New York, N.Y. : 1994)     Volume:  5     ISSN:  1073-9688     ISO Abbreviation:  Microcirculation     Publication Date:  1998  
Date Detail:
Created Date:  1999-03-29     Completed Date:  1999-03-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9434935     Medline TA:  Microcirculation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  265-74     Citation Subset:  IM    
Center for Biomedical Engineering, University of Kentucky, Lexington 40506-0070, USA.
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MeSH Terms
Albumins / metabolism
Biological Transport
Endothelium, Vascular / metabolism
Glycosaminoglycans / chemistry
Hyaluronic Acid / chemistry
Hyaluronoglucosaminidase / pharmacology
Isotonic Solutions / metabolism
Mesentery / blood supply,  drug effects,  physiology*
Osmotic Pressure
Grant Support
Reg. No./Substance:
0/Albumins; 0/Glycosaminoglycans; 0/Isotonic Solutions; 8022-63-7/Ringer's lactate; 9004-61-9/Hyaluronic Acid; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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