Document Detail


Effect of the flavonoid quercetin on cadmium-induced hepatotoxicity.
MedLine Citation:
PMID:  18433971     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study was designed to evaluate whether treatment with quercetin exerts any beneficial effect on cadmium (Cd)-induced hepatotoxicity in order to establish the possible protective mechanisms of quercetin. Wistar rats were distributed in four experimental groups: control, Cd, quercetin, and Cd+quercetin. Hepatic toxicity was evaluated by measuring plasma concentrations of markers of hepatic injury. The activity of antioxidant enzymes in liver was also measured. Hepatic expression of metallothioneins (MT), and endothelial nitric oxide synthase (eNOS) was assayed by Western and Northern blot. Our results demonstrated that Cd administration induced an increased marker enzyme activity in plasma. This effect was not inhibited by quercetin. However, the administration of quercetin softened Cd-induced oxidative damage. MT levels in liver were substantially increased when the animals received Cd and quercetin. Hepatic eNOS expression was significantly increased after treatment with Cd and quercetin, being this increase higher than in animals receiving Cd alone. In conclusion, in this experimental model, quercetin was not able to prevent the Cd-induced liver damage although the animals that received both, Cd and quercetin showed a marked improvement in oxidative stress and an increase in the MT and eNOS expression. These results suggest that other mechanisms different to oxidative stress could be involved in hepatic damage.
Authors:
C Vicente-Sánchez; J Egido; P D Sánchez-González; F Pérez-Barriocanal; J M López-Novoa; A I Morales
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-14
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  46     ISSN:  0278-6915     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-09     Completed Date:  2008-07-10     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2279-87     Citation Subset:  IM    
Affiliation:
Instituto Reina Sofía de Investigación Nefrológica, Departamento de Fisiología y Farmacología, Universidad de Salamanca, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / blood
Blotting, Northern
Blotting, Western
Cadmium / metabolism
Cadmium Poisoning / pathology*,  prevention & control*
Drug-Induced Liver Injury / pathology*,  prevention & control*
Liver / drug effects,  metabolism
Liver Function Tests
Male
Metallothionein / metabolism
Nitric Oxide Synthase Type III / biosynthesis
Oxidative Stress / drug effects
Quercetin / pharmacology*
RNA / biosynthesis,  genetics
Rats
Rats, Wistar
Thiobarbituric Acid Reactive Substances / metabolism
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Thiobarbituric Acid Reactive Substances; 117-39-5/Quercetin; 63231-63-0/RNA; 7440-43-9/Cadmium; 9038-94-2/Metallothionein; EC 1.14.13.39/Nitric Oxide Synthase Type III

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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