Document Detail


Effect of fish oil and sunflower oil on rumen fermentation characteristics and fatty acid composition of digesta in ewes fed a high concentrate diet.
MedLine Citation:
PMID:  20855014     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Studies in ruminants have shown that supplementing the diet with a mixture of fish oil (FO) and sunflower oil (SO) enhances the concentration of cis-9, trans-11 conjugated linoleic acid (CLA), 20:5 n-3, and 22:6 n-3 in milk because of alterations in ruminal biohydrogenation, but the intermediates formed under these conditions are not well characterized. Five ewes fitted with rumen cannula and fed a high concentrate diet were used to examine the effect of a mixture (30 g/kg of DM) of FO and SO (1:2, wt/wt) on temporal changes in rumen fermentation characteristics and the relative abundance of biohydrogenation intermediates in ruminal digesta collected after 0, 3, and 10 d on diet. Appearance and identification of biohydrogenation intermediates was determined based on complementary gas-liquid chromatography and Ag+-HPLC analysis of fatty acid methyl esters and gas chromatography-mass spectrometry analysis of corresponding 4,4-dimethyloxazoline derivatives. Inclusion of FO and SO in the diet had no effect on rumen pH, volatile fatty acid concentrations, or nutrient digestion, but altered the fatty acid composition of ruminal digesta, changes that were characterized by time-dependent decreases in 18:0 and 18:2 n-6 and the accumulation of trans 16:1, trans 18:1, 10-O-18:0, and trans 18:2. Lipid supplements enhanced the proportion of 20:5 n-3 and 22:6 n-3 in digesta and resulted in numerical increases in cis-9, trans-11 conjugated linoleic acid concentrations, but decreased the relative abundance of trans-10, cis-12 conjugated linoleic acid. Furthermore, detailed analysis revealed the appearance of several unique 20:1, 20:2, 22:1, 22:3, and 22:4 products in ruminal digesta that accumulated over time, providing the first indications of 20 and 22 carbon fatty acid intermediates formed during the biohydrogenation of long-chain unsaturated fatty acids in sheep. In conclusion, FO and SO in a high concentrate diet caused a time-dependent inhibition of the complete biohydrogenation of 16 and 18 carbon unsaturated fatty acids, and resulted in the accumulation of trans 16:1, trans 18:1, and trans 18:2, 20, and 22 carbon metabolites in ruminal digesta of sheep, with no evidence of a shift in ruminal biohydrogenation pathways toward trans-10 18:1 formation.
Authors:
P G Toral; K J Shingfield; G Hervás; V Toivonen; P Frutos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of dairy science     Volume:  93     ISSN:  1525-3198     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-21     Completed Date:  2010-12-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4804-17     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.
Affiliation:
Instituto de Ganadería de Montaña (CSIC-Universidad de León), Finca Marzanas s/n, 24346 Grulleros, León, Spain. pg.toral@eae.csic.es
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MeSH Terms
Descriptor/Qualifier:
Animal Feed
Animal Nutritional Physiological Phenomena
Animals
Diet / veterinary*
Dietary Supplements*
Fatty Acids / analysis*
Female
Fermentation
Fish Oils / administration & dosage*
Linoleic Acids, Conjugated / analysis
Milk / chemistry
Plant Oils / administration & dosage*
Rumen / metabolism*
Sheep / metabolism*
Time Factors
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Fish Oils; 0/Linoleic Acids, Conjugated; 0/Plant Oils; 0/cis-9, trans-11-conjugated linoleic acid; 8001-21-6/sunflower seed oil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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