| Effect of feeding a high protein diet on amino acid uptake into rat intestinal brush border membrane vesicles. | |
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MedLine Citation:
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PMID: 6709488 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Uptake of the neutral amino acid L-leucine into isolated rat intestinal brush border membrane (= BBM) vesicles and into a jejunal mucosa preparation as affected by the protein content of the diet was investigated. Adult rats fed either a high carbohydrate (HC) diet (11% protein) or a high protein (HP) diet (77% protein) for several weeks were used for the experiments. The time course of L-leucine uptake into BBM vesicles prepared from the small intestine of HC- or HP-rats was studied under conditions of an inwardly directed Na+-gradient and under Na+-equilibrium conditions. Furthermore, in one series of experiments the Na+-equilibrium was replaced by a K+-equilibrium. L-leucine uptake under Na+-gradient conditions displayed the overshoot phenomenon typically associated with Na+-gradient-dependent active transport processes in BBM vesicles and the overshoot in group HP exceeded that in group HC significantly. Under both Na+- and K+-equilibrium conditions L-leucine uptake into the BBM-vesicles also was faster in group HP. Finally L-leucine uptake into jejunal mucosa in group HP exceeded that in group HC, too. The results therefore indicate that Na+-dependent and Na+-independent transport of neutral amino acids across the intestinal brush border membrane adapts to the dietary protein level. |
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Authors:
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S Wolffram; E Scharrer |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pflügers Archiv : European journal of physiology Volume: 400 ISSN: 0031-6768 ISO Abbreviation: Pflugers Arch. Publication Date: 1984 Jan |
Date Detail:
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Created Date: 1984-05-02 Completed Date: 1984-05-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0154720 Medline TA: Pflugers Arch Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 34-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Dietary Carbohydrates / pharmacology Dietary Proteins / pharmacology* Glucose / metabolism Intestines / metabolism, ultrastructure* Leucine / metabolism* Male Microvilli / metabolism Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
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0/Dietary Carbohydrates; 0/Dietary Proteins; 50-99-7/Glucose; 61-90-5/Leucine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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