Document Detail


Effect of fatty acids on herbicide transport across Caco-2 cell monolayers.
MedLine Citation:
PMID:  15896553     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oral ingestion of pesticides can be a major exposure route. These compounds are frequently consumed in the presence of triacylglycerides, which are then hydrolyzed to free fatty acids. The purpose of this work was to examine the effect of two common fatty acids, palmitic (PA) and oleic (OA) acids, and the biological emulsifier sodium taurocholate (TC) on the absorption of three herbicides (trifluralin, alachlor and atrazine) by Caco-2 cell monolayers. Trifluralin's absorption was enhanced (p < 0.05) in the presence of OA whereas the greatest absorption of atrazine and alachlor occurred with PA and the control media, respectively. Trifluralin had significantly lower absorption through the monolayer than either alachlor or atrazine (p < 0.001). A mass balance study demonstrated that trifluralin accumulated within the cell monolayer (13.85% of the donor after 3 h of exposure), but alachlor and atrazine (1.27% and 0.85%, respectively) did not. This response was linear with time (21.89% trifluralin after 6 h of exposure), and demonstrated the potential for continued release of trifluralin after source removal. These experiments demonstrated that fatty acids and an emulsifier can influence absorption of herbicides across small intestinal epithelium.
Authors:
Rhonda M Brand; Yuksel Cetin; Cynthia Mueller; Susan L Cuppett
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  19     ISSN:  0887-2333     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-05-17     Completed Date:  2005-08-18     Revised Date:  2009-04-10    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  595-601     Citation Subset:  IM    
Affiliation:
Division of Emergency Medicine of Evanston, Northwestern Healthcare and Department of Medicine, Fienberg School of Medicine at Northwestern University, Evanston, IL 60201, USA. rhbrand@enh.org
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MeSH Terms
Descriptor/Qualifier:
Acetamides / pharmacokinetics
Atrazine / pharmacokinetics
Biological Transport / drug effects
Caco-2 Cells
Herbicides / pharmacokinetics*
Humans
Intestinal Absorption / drug effects*
Oleic Acid / pharmacology*
Palmitic Acid / pharmacology*
Trifluralin / pharmacokinetics
Chemical
Reg. No./Substance:
0/Acetamides; 0/Herbicides; 112-80-1/Oleic Acid; 1582-09-8/Trifluralin; 15972-60-8/alachlor; 1912-24-9/Atrazine; 57-10-3/Palmitic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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