Document Detail

Effect of fatty acids and cholesterol present in bile on expression of virulence factors and motility of Vibrio cholerae.
MedLine Citation:
PMID:  17261615     Owner:  NLM     Status:  MEDLINE    
Bile induces pleiotropic responses that affect production of virulence factors, motility, and other phenotypes in the enteric pathogen Vibrio cholerae. Since bile is a heterogeneous mixture, crude bile was fractionated, and the components that mediate virulence gene repression and enhancement of motility were identified by nuclear magnetic resonance, gas chromatography (GC), and GC-mass spectrometry analyses. The unsaturated fatty acids detected in bile, arachidonic, linoleic, and oleic acids, drastically repressed expression of the ctxAB and tcpA genes, which encode cholera toxin and the major subunit of the toxin-coregulated pilus, respectively. The unsaturated fatty acid-dependent repression was due to silencing of ctxAB and tcpA expression by the histone-like nucleoid-structuring protein H-NS, even in the presence of the transcriptional activator ToxT. Unsaturated fatty acids also enhanced motility of V. cholerae due to increased expression of flrA, the first gene of a regulatory cascade that controls motility. H-NS had no role in the fatty acid-mediated enhancement of motility. It is likely that the ToxR/ToxT system that negatively regulates motility is rendered nonfunctional in the presence of unsaturated fatty acids, leading to an increase in motility. Motility and flrA expression were also increased in the presence of cholesterol, another component of bile, in an H-NS- and ToxR/ToxT-independent manner.
Arpita Chatterjee; Pradeep K Dutta; Rukhsana Chowdhury
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-29
Journal Detail:
Title:  Infection and immunity     Volume:  75     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-22     Completed Date:  2007-05-03     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1946-53     Citation Subset:  IM    
Biophysics Division, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Calcutta 700 032, India.
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MeSH Terms
Anti-Bacterial Agents / pharmacology*
Bacterial Proteins / antagonists & inhibitors,  biosynthesis,  drug effects
Bile / chemistry*
Cholera Toxin / biosynthesis,  genetics
Cholesterol / isolation & purification,  pharmacology*
DNA-Binding Proteins / antagonists & inhibitors,  drug effects
Fatty Acids, Unsaturated / isolation & purification,  pharmacology*
Fimbriae Proteins / biosynthesis,  genetics
Gas Chromatography-Mass Spectrometry
Gene Expression / drug effects
Magnetic Resonance Spectroscopy
Movement / drug effects
Vibrio cholerae / drug effects*,  genetics,  physiology
Virulence Factors / antagonists & inhibitors,  biosynthesis*
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bacterial Proteins; 0/DNA-Binding Proteins; 0/Fatty Acids, Unsaturated; 0/H-NS protein, bacteria; 0/TcpA protein, Vibrio cholerae; 0/Virulence Factors; 147680-16-8/Fimbriae Proteins; 57-88-5/Cholesterol; 9012-63-9/Cholera Toxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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