Document Detail


Effect of extracellular glucose and K+ on intracellular osmolytes and volume in a human kidney cell line.
MedLine Citation:
PMID:  17485379     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The goal of this study was to assess the effect of extracellular glucose and K+ ((K)o) on the intracellular osmolyte content and cell volume maintenance and regulation in a human embryonic kidney cell line (tsA201a). Cell volume maintenance was studied by isotonic (313 +/- 5 mOsm) replacement of culture media by a glucose-free Ringer solution containing (in mM) 0, 3, 6, or 10 K+. Cell volume regulation was studied by exposing cells to hypotonic (250 +/- 5 mOsm) glucose-free Ringer solution containing the various (K)o. The results showed that: 1) intracellular osomlyte content (i.e. Na+, Cl-, Urea and free amino acids (FAA)) and cell volume increased when culture media was replaced with isotonic Ringer at all (K)o; 2) osmolyte content decreased with continuous exposure to isotonic Ringer at all (K)o but cell volume changes depended on (K)o. Volume recovery occurred at 6 and 10 mM K+; 3) exposure to hypotonic Ringer induced swelling at all (K)o followed by a reduction in measured intracellular osmolytes. Regulatory volume decrease occurred in 6 or 10 mM K+ but swelling continued in 0 or 3 mM K+; and 4) addition of ouabain produced swelling without recovery under iso- and hypotonic conditions. These results indicate that the removal of extracellular glucose produced a transient inhibition of the Na+/K+ ATPase resulting in a transient increase in the intracellular content of Urea, FAA and cell volume and (K)o regulated an as yet unidentified intracellular osmolyte.
Authors:
Marcy D Hubert; Elisabeth Indyk; Cecilia Pena-Rasgado; Sydney K Pierce; Hector Rasgado-Flores; Sarah S Garber
Related Documents :
1556089 - Staphylococcus aureus osmoregulation: roles for choline, glycine betaine, proline, and ...
8699159 - Salinity change and cell volume: the response of tissues from the estuarine mussel geuk...
6292509 - Poliovirus-induced alterations in hela cell membrane functions.
6353219 - A nuclear mutation decreasing rho- production modifies the unstable nitrous-acid sensit...
9644219 - Cellular taurine release triggered by stimulation of the fas(cd95) receptor in jurkat l...
2918039 - Ionic dependence of the extracellular atp-induced permeabilization of transformed mouse...
23484119 - Reevaluating the role of acanthamoeba proteases in tissue invasion: observation of cyto...
2993719 - Sources of extramitochondrial corticoidogenic cholesterol in the adrenal cortex.
15070739 - Partially redundant functions of src-1 and tif2 in postnatal survival and male reproduc...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-05-01
Journal Detail:
Title:  Frontiers in bioscience : a journal and virtual library     Volume:  12     ISSN:  1093-4715     ISO Abbreviation:  Front. Biosci.     Publication Date:  2007  
Date Detail:
Created Date:  2007-05-08     Completed Date:  2007-06-29     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  9709506     Medline TA:  Front Biosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4352-61     Citation Subset:  IM    
Affiliation:
Dept. of Physiology and Biophysics, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Line
Cell Size
Extracellular Space / metabolism
Fluorescence
Glucose / metabolism*
Humans
Kidney / cytology,  metabolism*
Potassium / metabolism*
Grant Support
ID/Acronym/Agency:
NIDDK46672//PHS HHS
Chemical
Reg. No./Substance:
50-99-7/Glucose; 7440-09-7/Potassium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Tetraplex DNA and its interacting proteins.
Next Document:  Induction of autoimmunity after allotransplantation.