Document Detail

Effect of extracellular glucose and K+ on intracellular osmolytes and volume in a human kidney cell line.
MedLine Citation:
PMID:  17485379     Owner:  NLM     Status:  MEDLINE    
The goal of this study was to assess the effect of extracellular glucose and K+ ((K)o) on the intracellular osmolyte content and cell volume maintenance and regulation in a human embryonic kidney cell line (tsA201a). Cell volume maintenance was studied by isotonic (313 +/- 5 mOsm) replacement of culture media by a glucose-free Ringer solution containing (in mM) 0, 3, 6, or 10 K+. Cell volume regulation was studied by exposing cells to hypotonic (250 +/- 5 mOsm) glucose-free Ringer solution containing the various (K)o. The results showed that: 1) intracellular osomlyte content (i.e. Na+, Cl-, Urea and free amino acids (FAA)) and cell volume increased when culture media was replaced with isotonic Ringer at all (K)o; 2) osmolyte content decreased with continuous exposure to isotonic Ringer at all (K)o but cell volume changes depended on (K)o. Volume recovery occurred at 6 and 10 mM K+; 3) exposure to hypotonic Ringer induced swelling at all (K)o followed by a reduction in measured intracellular osmolytes. Regulatory volume decrease occurred in 6 or 10 mM K+ but swelling continued in 0 or 3 mM K+; and 4) addition of ouabain produced swelling without recovery under iso- and hypotonic conditions. These results indicate that the removal of extracellular glucose produced a transient inhibition of the Na+/K+ ATPase resulting in a transient increase in the intracellular content of Urea, FAA and cell volume and (K)o regulated an as yet unidentified intracellular osmolyte.
Marcy D Hubert; Elisabeth Indyk; Cecilia Pena-Rasgado; Sydney K Pierce; Hector Rasgado-Flores; Sarah S Garber
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-05-01
Journal Detail:
Title:  Frontiers in bioscience : a journal and virtual library     Volume:  12     ISSN:  1093-4715     ISO Abbreviation:  Front. Biosci.     Publication Date:  2007  
Date Detail:
Created Date:  2007-05-08     Completed Date:  2007-06-29     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  9709506     Medline TA:  Front Biosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4352-61     Citation Subset:  IM    
Dept. of Physiology and Biophysics, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064, USA.
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MeSH Terms
Cell Line
Cell Size
Extracellular Space / metabolism
Glucose / metabolism*
Kidney / cytology,  metabolism*
Potassium / metabolism*
Grant Support
Reg. No./Substance:
50-99-7/Glucose; 7440-09-7/Potassium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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