Document Detail

Effect on expression of RT1-A and RT1-DM molecules of treatment with interferon-gamma at the maternal--fetal interface of pregnant rats.
MedLine Citation:
PMID:  15946996     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Recent studies suggest a role for interferon-gamma (IFN-gamma) in the pregnancy process. METHODS: The expression of non-classical class II major histocompatibility complex (RT1-DM) antigens and classical class I major histocompatibility complex (RT1-A) antigens induced by IFN-gamma was examined by reverse transcription-PCR, western blotting and immunohistochemistry. RESULTS: IFN-gamma treatment increased expression of RT1-DM and RT1-A during early pregnancy and decreased them during mid pregnancy at the maternal-fetal interface. In late pregnancy, expression of RT1-A decreased in placenta and increased in uterus, and RT1-DM increased in both placenta and uterus with IFN-gamma treatment compared with untreated controls. Immunohistochemical studies suggested that in early pregnancy, RT1-DM protein mainly localized to uterine luminal epithelium and glandular epithelium, and RT1-A mainly localized to decidual blood vessels and decidua basalis. During mid and late pregnancy, RT1-A mainly localized in decidual blood vessels and spongiotrophoblast cells of the junction zone. RT1-DM mainly localized in blood vessels and the labyrinthine zone during mid and late gestation. CONCLUSIONS: RT1-A and RT1-DM can both be expressed at the maternal-fetal interface during normal pregnancy. Their localizaion changed according to the period of pregnancy. IFN-gamma can modulate the expression of these two molecules during the whole pregnancy.
Quan-Hong Sun; Jing-Pian Peng; Hong-Fei Xia; Ying Yang; Mei-Ling Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-06-09
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  20     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-08-11     Completed Date:  2005-10-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  2639-47     Citation Subset:  IM    
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing.
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MeSH Terms
Decidua / drug effects,  immunology*,  metabolism
Gene Expression / drug effects,  immunology
Histocompatibility Antigens / genetics*,  metabolism
Interferon-gamma / pharmacology,  physiology*
Maternal-Fetal Exchange / immunology
RNA, Messenger / analysis
Rats, Sprague-Dawley
Reg. No./Substance:
0/Histocompatibility Antigens; 0/RNA, Messenger; 0/histocompatibility antigens RT, rat; 82115-62-6/Interferon-gamma

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