| Effect of exogenous hydrogen peroxide on biophoton emission from radish root cells. | |
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MedLine Citation:
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PMID: 20106674 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Biophotons spontaneously emitted from radish root cells were detected using highly sensitive photomultiplier tube. Freshly isolated radish root cells exhibited spontaneous photon emission of about 4 counts s(-1). Addition of hydrogen peroxide to the cells caused significant enhancement in biophoton emission to about 500 counts s(-1). Removal of molecular oxygen using glucose/glucose oxidase system and scavengering of reactive oxygen species by reducing agents such are sodium ascorbate and cysteine completely diminished biophoton emission. Spectral analysis of the hydrogen peroxide-induced biophoton emission indicates that biophotons are emitted mainly in green-red region of the spectra. The data provided by electron paramagnetic resonance spin-trapping technique showed that formation of singlet oxygen observed after addition of H2O2 correlates with enhancement in biophoton emission. These observations provide direct evidence that singlet oxygen is involved in biophoton emission from radish root cells. |
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Authors:
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Anshu Rastogi; Pavel Pospísil |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-11 |
Journal Detail:
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Title: Plant physiology and biochemistry : PPB / Société française de physiologie végétale Volume: 48 ISSN: 1873-2690 ISO Abbreviation: Plant Physiol. Biochem. Publication Date: 2010 Feb-Mar |
Date Detail:
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Created Date: 2010-03-09 Completed Date: 2010-09-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9882449 Medline TA: Plant Physiol Biochem Country: France |
Other Details:
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Languages: eng Pagination: 117-23 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Masson SAS. All rights reserved. |
Affiliation:
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Department of Experimental Physics, Laboratory of Biophysics, Faculty of Science, Palacký University, tr. 17. listopadu 1192/12, 771 46 Olomouc, Czech Republic. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Electron Spin Resonance Spectroscopy Free Radical Scavengers Hydrogen Peroxide / pharmacology* Luminescence* Oxygen / chemistry* Photons* Plant Roots / chemistry* Raphanus / chemistry* Reactive Oxygen Species* Reducing Agents / pharmacology Spectrum Analysis Spin Trapping |
| Chemical | |
Reg. No./Substance:
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0/Free Radical Scavengers; 0/Reactive Oxygen Species; 0/Reducing Agents; 7722-84-1/Hydrogen Peroxide; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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